摘要
目的:初步探讨信号素3A(Semaphorin3A,Sema3A)对成牙骨质细胞增殖、矿化的影响。方法:采用不同浓度的重组人Sema3A(0、0.5、1mg/L)处理成牙骨质细胞OCCM-30。MTT法检测Sema3A对成牙骨质细胞增殖的影响,酶比活性法检测细胞碱性磷酸酶(ALP)活性的变化,实时荧光定量PCR法检测骨钙素(OCN),骨桥蛋白(OPN)和Runx2基因的表达,茜素红染色法检测矿化结节的形成。结果:Sema3A对成牙骨质细胞的增殖和ALP活性无明显影响。Sema3A抑制OCN和Runx2基因的表达(P<0.05),并抑制矿化结节的形成。结论:Sema3A对成牙骨质细胞的增殖无明显影响,但对细胞的矿化功能起到抑制作用。
Objective:To investigate the effects of Semaphorin3A(Sema3A)on the proliferation and mineralization of cementoblasts.Methods:The immortalized murine cementoblast cell line OCCM-30 was treated with recombinant human Sema3A(0,0.5,1mg/L).Cell proliferation was tested by MTT assay.Alkaline phosphotase(ALP)activity was detected using an ALP kit.The mRNA levels of osteocalcin(OCN),osteopontin(OPN)and Runx2 were investigated by quantitative reverse transcription PCR(RT-PCR).Calcium deposition was assayed with Alizarin red staining(ARS).Results:The proliferation and the ALP activity of OCCM-30 treated with Sema3 A were not significantly different from those of the control.The OCN and Runx2 mRNA expressions of the OCCM-30 treated with Sema3 Asignificantly declined in contrast to that of the control.Sema3 Aalso inhibited the mineral nodule formation in vitro compared to untreated cells.Conclusion:Sema3Adid not obviously affect the proliferation,but suppressed the mineralization of the cementoblasts.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2016年第5期445-448,共4页
Journal of Oral Science Research
基金
重庆市自然科学基金资助项目(CSTC
2011jjA10086)
重庆市渝中区科委资助项目(20140128)
2013年重庆高校创新团队建设计划资助项目
关键词
信号素3A
成牙骨质细胞
增殖
矿化
Semaphorin3A
Cementoblast
Proliferation
Mineralization