应用ELISA法检测重症肌无力患者血清中胸腺瘤相关抗体

Applying ELISA to detect thymoma-asso-ciated antibody in the sera of myasthe-nia gravis.

本文应用ELISA法检测123例重症肌无力(MG)患者血清中骨骼肌柠檬酸提取物抗体(CAE-Ab)。观察到MG合并胸腺瘤的阳性率为82.6％(19/23),略高于间接血凝法(IHA),而MG无胸腺瘤的检出率为4.0％(3/77)。结果表明CAE-Ab与MG合并胸腺瘤有显著相关性。ELISA法免去醛化、鞣化及抗原致敏绵羊红细胞等繁杂步骤,方法简便,更容易推广使用,可协助临床发现胸腺瘤。

An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies of citric acid extract of skeletal muscle (CAE-Ab) in sera from 123 cases with myasthenia gravis (MG) was described. It was shown that CAE-Ab was detected in nineteen of 23 sera MG patients with thymoma, who have been verified by operative biopsy. The positive rate was 82.6%, which was slightly higher than that in indirect hemoagglu-tination assay (IHA), but the positive rate of MG without thymoma was only 4.0% (3/77). It was indicated that there was a significant interrelationship between CAE-Ab and the MG patients with thymoma. Omitting the complex procedures such as aldehyding, tanning and antigen sensitivating to the erythrocyts, this assay method was simplified and was easy to apply widely, and contributed to find thymoma.