AKT、ATM、D4-GDI和p53 4条细胞凋亡通路基因在大鼠肝再生过程中的表达变化

Expression changes of AKT,ATM,D4-GDI and p53 cell apoptosis pathway genes in the process of rat liver regeneration

目的探讨AKT、ATM、D4-GDI和p53 4条细胞凋亡通路基因在肝再生(LR)过程中的表达变化。方法将大鼠随机分为实验组和对照组,每组6只,雌雄各半,用Rat Genome 230 2.0芯片检测大鼠部分肝切除(PH)后不同恢复时间点4条细胞凋亡通路基因表达情况,并用生物信息学方法对其进行分析。结果 AKT、ATM、D4-GDI和p53 4条细胞凋亡通路中63、8、6和7个基因与肝再生相关。它们主要在肝再生启动阶段起始表达。表达的相似性分为均上调、上调占优势、均下调、下调占优势、上调和下调相近等5类,大多数基因表达加强,少数基因表达降低。它们表达的时间相关性分为11组。基因协同作用模型(Et)分析表明,AKT通路几乎在整个肝再生中抑制细胞凋亡;ATM、D4-GDI和p53 3条细胞凋亡通路几乎在整个肝再生中促进细胞凋亡。结论 AKT、ATM、D4-GDI和p53 4条细胞凋亡通路与再生肝生长、发育和肝量控制密切相关。

Objective To investigate the expression changes of four cell apoptosis pathway genes,AKT,ATM,D4-GDI and p53 in the process of liver regeneration. Methods The rats were randomly divided into enperiment group and control group,6 rats in each group,half male and half female. Genome Rat 230 2. 0 was used to detect the expression of genes in four cell apoptosis pathways in rats after partial hepatectomy（ PH）,and the biological information method was used to analyze it. Results 63,8,6 and 7 genes were associated with liver regeneration in the four cell apoptosis pathways. They were mainly expressed at the start stage of liver regeneration. The similarity of their expression was including totally up regulation,up regulation advantage,totally down regulation,down regulation advantage,up regulation equal to down regulation of 5 categories. The expression of most genes in liver regeneration was enhanced,and the expression of few genes was decreased. Time correlation was divided into 11 groups. Analysis of the role of apoptosis pathway related genes in liver regeneration by using the gene synergy model（ Et） indicated that AKT pathway inhibited the apoptosis of cells in the whole liver regeneration; ATM,D4-GDI and p53 three cell apoptosis pathway in the promotion of cell apoptosis in the whole liver regeneration. Conclusion Four apoptosis pathways,AKT,ATM,D4-GDI and p53,are closely related to the growth,development,and liver volume control of liver regeneration.