兔带蒂皮瓣血供变化及再血管化的实验研究

Experimental Study of Changes in Blood Supply and Revascularization of Pedicled Skin Flap in Rabbit

新西兰兔66只,随机分8组,观察Ⅰ型(含侧背部纵行血管)和Ⅱ型(不含纵行血管)皮瓣的术后血供变化和再血管化。用99mTc清除法检测皮瓣术后血供变化,而皮瓣再血管化则用荧光素试验和99mTc清除法检测,组织透明法了解皮瓣与受区血管速接方式和时间。观察结果:Ⅱ型皮瓣术后10天完全再血管化,而Ⅰ型则术后14天。皮瓣缺血是促进再血管化的因素。

This experiment was to study the blood supply of different types of skin flaps and the revascularization of the host region. The types of skin flaps used in this experiment were the axial pattern type (Type Ⅰ) and the random pattern type (Type Ⅱ). The two pattern types were self-matched on the back of rabbit. Sixty-six New Zealand rabbits were divided into 8 groups at random. The first group, 10 rabbits, was the group to observe the changes in blood supply and the other 7 groups, each of 8 rabbits, were used to observe the nature of revascularization of the two patterns. In gathering the data for assessment, besides the local changes such as color, swelling of the flaps, the area of survival of flaps after division of the pedicles, the tests used to observe the postoperative changes included the isotope (99mTc) clearance test, intravenous fluorescein test and tissue transparent method by perfusion of the flap vessels with chengdu ink. The following conclusions could be drawn, The complete revascularization in Type Ⅱ occurred at 10 days after operation and that of Type Ⅰ was 14 days, Type Ⅱ skin flaps had a quicker rate of revascularization. It suggested that flap ischemia was a factor enhanced revascularization.