酸敏感离子通道在紫癜性肾炎肾组织中的表达及其对肾损伤的影响

The expression and role of acid-sensing ion channels in renal tissues from patients with Henoch Schnlein purpura nephritis

目的观察酸敏感离子通道(acid-sensing ion channels,ASICs)在紫癜性肾炎(henoch-Schnlein purpura nephritis,HSPN)患儿肾组织中的表达并探讨其在HSPN患儿肾损伤中的作用。方法采用免疫组化法观察HSPN患儿及单纯肾病综合征患儿肾组织中ASIC1a、ASIC2a、ASIC3的表达部位及水平;对HSPN患儿24 h尿蛋白定量与肾组织中ASICs的表达做相关性分析,另外根据尿系列蛋白检查中的αl-MG和β2-MG是否异常将HSPN患儿分为两组,观察ASICs在其两组HSPN患儿肾小管中的表达情况。结果免疫组化显示ASIC1a、ASIC2a、ASIC3在HSPN患儿肾小管上的表达较对照组显著增多(P<0.01)。ASIC3在HSPN患儿肾小管上的表达定量与24 h尿蛋白定量呈正相关(r=0.644,P=0.017),根据尿系列蛋白检查αl-MG和β2-MG是否异常将HSPN患儿分为两组,ASIC3在αl-MG异常组和β2-MG异常组中的表达较对照组(正常组)显著增多(P<0.01),ASIC2a在αl-MG异常组中的表达较对照组(正常组)增多(P<0.05)。结论 ASICs可能参与了紫癜性肾炎肾小管的损伤过程。ASICs与HSPN患儿蛋白尿,尤其是微量蛋白尿的产生可能存在一定关系。

Objective To observe the expression of acid -sensing ion channels （ASICs） in renal tissues from patients with Henoch -Sch·nlein purpura nephritis （HSPN） and to explore the action and mechanism of ASICs in the inflammatory injury of renal tissues. Methods Immunohistochemical methods were used to investigate the expression and localization of ASICs in renal tissue from patients with HSPN and patients with dropsical nephritis.The expressions of ASICs were semiquantitatively assessed by the percentage of posi-tive area of ASIC1a, ASIC2a, and ASIC3.The correlation between expression of ASICs and quantity of 24 -hour urinary protein was analyzed.According to different expressions of α1 -MG and β2 -MG in the test of urine trace protein, patients with HSPN were divid-ed into two groups.The expressions of ASICs in renal tubules of those two groups were assessed respectively.Results Immunohisto-chemical analysis revealed that higher expression of ASIC1a, ASIC2a and ASIC3 was noted in renal tubules from patients with HSPN. The expression of ASIC3 in patients with HSPN was positively correlated with quantity of 24 -hour urinary protein.Compared with the group with normal expressions of α1 -MG and β2 -MG, the expression of ASIC3 was significantly increased in the group with abnormal expressions of α1 -MG and β2 -MG （P 〈0.01） and higher expression of ASIC2a was noted in the group with abnormal expressions ofα1 -MG （P 〈0.01）.Conclusions This study suggested an excitive role of ASICs in the pathogenesis of the injury of renal tubules in patients with HSPN.The expression of ASICs may be related to the production of proteinuria, especially microalbuminuria.