miR-506对胃癌细胞增殖和转移的影响

Effects of miR-506 on the proliferation and metastasis in gastric cancer cells

目的探讨mi R-506对胃癌细胞增殖和转移的抑制作用。方法采用实时荧光定量PCR检测5株胃癌细胞系(MKN-45、SGC-7901、BGC-823、NCI-N87和AGS)中mi R-506的表达量,以永生化的正常胃黏膜上皮细胞GES-1为对照。通过脂质体转染的方法在SGC-7901细胞系中瞬时转染mi R-506和anti-miR-506上调和下调mi R-506表达,通过CCK-8实验观察细胞增殖能力改变,通过划痕实验、Transwell迁移和侵袭实验观察细胞运动迁移和侵袭能力的变化。结果 miR-506在各胃癌细胞中的表达量相对于正常胃黏膜上皮细胞GES-1显著降低(P<0.05)。过表达mi R-506可显著抑制SGC-7901细胞的增殖、迁移和侵袭能力,与阴性对照的差异有统计学意义(P<0.01)。抑制mi R-506表达则可促进增殖、迁移和侵袭能力(P<0.01)。结论胃癌细胞系中mi R-506明显降低,上调mi R-506可以抑制胃癌细胞的增殖、迁移和侵袭能力,下调则逆转上述过程。

Objective To investigate the effects of miR-506 on inhibiting the proliferation and metastasis in gastric cancer cells. Methods The miR-506 expressions in five gastric cancer cell lines（ MKN-45, SGC-7901,BG C-823, NCI-N87, and AG S） were measured with real-time fluorescent quantitative PC R. The im ortalized human normal gastric mucosa epithelial cell line G ES-1 was served as control. MiR-506 mimics and anti-miR-506 were transiently transfected into SG C-7901 cell line to up-regulate and dow n-regulate the miR-506 expression with liposom e transfection method. Changes in the proliferation of SG C-7901 were observed w ith C C K-8, while cell migration and invasion abilities w ere observed with wound healing assay and Transw ell migration and invasion assay. Results T he expressions of m i R-506 in five gastric cancer cell lines were significantly lower as com pared with norm al gastric mucosa epithelial cell line GES-1（ P 〈0. 05）. MiR-506 over-expression could significantly inhibit the proliferation, migration, and invasion abilities in SGC-7901 cells. Com pared with negative controls, the difference was statistically significant（ P 〈0. 01）. T he inhibition of mi R-506 expression could promote cell proliferation, migration, and invasion abilities（ P 〈0. 01）. Conclusion T he expression of mi R-506 significantly decreases in gastric cancer cell lines. Up-regulation of mi R-506 can inhibit proliferation, migration,and invasion abilities in gastric cancer cells, while dow n-regulation of miR-506 has the opposite effects.