DHA对P.gingivalis LPS诱导人牙周膜成纤维细胞炎症反应的影响

Effects of DHA on inflammatory response in human periodontal ligament cells induced by P. gingivalis LPS

目的观察二十二碳六烯酸（DHA）对牙龈卟啉单胞菌（P.gingivalis）脂多糖（LPS）诱导人牙周膜成纤维细胞（h PDLCs）表达炎症因子的影响。方法体外培养鉴定h PDLCs,采用MTT法观察不同浓度DHA对h PDLCs细胞活性的影响。根据MTT法的结果,选择对细胞活性没有影响的刺激浓度,分别采用real-time PCR和ELISA技术从基因和蛋白水平检测DHA对P.gingivalis LPS诱导h PDLCs表达炎症因子白细胞介素-6（IL-6）、IL-8和IL-1β的影响。结果在25～100μmol/L浓度范围内,DHA对h PDLCs细胞活性没有影响,且可呈浓度依赖性下调P.gingivalis LPS诱导h PDLCs表达的炎症因子。结论在不影响细胞活性的情况下,DHA可在一定浓度范围内,呈浓度依赖性下调P.gingivalis LPS诱导h PDLCs表达的炎症因子,表明DHA具有用于牙周炎抗炎治疗的可能性。

Objective To investigate the effects of docosahexaenoic acid（DHA） on periodontal ligament cells（ h PD LC s）-expressed inflam m atory factors induced by Porphyromonas gingivalis（ P. gingivalis） lipopolysaccharides（ LPS）. Methods H PD LC s were cultured and identified in vitro. The effects of different concentrations of DHA on the cell viability were detected with MTT assay. The concentrations with no effect on the cell viability were selected according to the results of M T T assay and the effects of DHA on h PD LC s-expressed inflam m atory factors interleukin-6（ IL-6）, IL-8, and IL-1β induced by P. gingivalis LPS were detected at gene and protein levels with real-tim e PC R and ELISA. Results In the range of 25- 100 μm ol / L, D H A had no effect on the cell viability in h PD LC s and down-regulated h PD LC s-expressed inflam m atory factors induced by P. gingivalis LPS in a concentration-dependent m anner. Conclusion In a certain range of concentrations, D H A can downregulated h PD LC s-expressed inflam m atory factors induced by P. gingivalis LPS in a concentration-dependent m anner without affecting the cell viability, suggesting the possibility of applying DHA to the anti-inflam m atory therapy of periodontosis.