利用二氧化钛静电纺丝和透明质酸甲基丙烯酸酯构筑用于细胞培养的纤维凝胶结构

Fabrication of Biocompatible Nanofibrious Scaffold by Electrospun TiO_2 and Methacrylated Hyaluronic Acid(MeHA)

通过观察生物体内骨的电镜图片,发现骨表面具有丰富的纤维微纳结构,在此观测的启发下,本实验设计将二氧化钛表面生物活性化的同时构筑这种纤维微纳结构,模仿骨表面。考虑到二氧化钛具有光催化性,且具有生物无毒性,本实验采用"自上而下"的一步法,在紫外光照的条件下,将二氧化钛静电纺丝与透明质酸衍生物在光照条件下通过双键稳定地连接起来。傅立叶-红外光谱分析表明实验成功地在二氧化钛静电纺丝表面嫁接上透明质酸分子。通过荧光和扫描电镜分析表明,间充质干细胞可以很好地在二氧化钛静电纺丝和透明质酸的复合结构上生长。这种构筑复合结构表面的方法,生物活化了二氧化钛纺丝的表面,同时模仿骨表面获得了微纳纤维拓扑结构。此外,可以将二氧化钛静电纺丝纺在不同种类的材料表面,从而在不同材料表面简便地得到可用于细胞培养的纤维表面结构,对于未来可实际应用的移植材料研发具有很好的借鉴意义。

By observing the electron microscopy images,it is found that the bone of the body has abundant fiber micro-nano structure.Inspired by this observation,we designed bio-activating surface of titanium dioxide electrospun and constructed the fiber micro-nano structure at the same time which imitated bone surface.Considering the titanum dioxide（TiO_2）electrospun possesses light-triggered property and non-toxic,we adopted the＂top-down＂one step method and synthesized MeHA to bio-activated the surfae of TiO_2 electrospun,which was obtained through uv-irradiation（UV）by a simple way.Fourier infrared spectrum analysis showed that the surface of titanum dioxide（TiO_2）electrospun grafted hyaluronic acid molecules successfully.By fluorescence and scanning electron microscopy（SEM）analysis,it shows that mesenchymal stem cells can growth well on the modified surface of TiO_2 electrospun.This building method bio-activated the surface of TiO_2 electrospun and imitated the bone surface through micro-nano fiber topology.The TiO_2 electrospun can spin on different kinds of materials surface,so the surfaces of different materials would easily get bio-activated fiber surface structure which can be used for cell culture.These results suggested that TiO_2 electrospun modified with MeHA are more suitable for implants and have large clinical potential.