补益营卫方对衰老皮肤表皮凋亡指数的影响

Effect of Supplemting Ying-Wei Formula on Apoptosis Rate of Skin Epidermis Aging

目的:观察补益营卫方对衰老小鼠皮肤表皮凋亡指数的影响,探讨补益营卫方延缓皮肤衰老的机制。方法:以自然衰老小鼠为研究对象,HE染色观察各组皮肤表皮形态学变化,应用TUNEL法和计算机图像分析系统对凋亡指数表达进行分析。结果:应用补益营卫方高、中、低剂量组处理后,可显著降低细胞凋亡,与老年组比较有统计学意义(P<0.05)。三个剂量组之间,中剂量组对凋亡抑制作用最大,较其他组有明显差异(P<0.01),低剂量组和高剂量组没有明显差异(P>0.05)。结论:补益营卫方通过抑制皮肤表皮细胞的凋亡,降低细胞凋亡指数,起到延缓皮肤衰老作用。

Objective： To discuss the mechanism of Supplemting Ying- Wei Formula on apoptosis rate of skin epidermis aging.Methods： The natural mice skin was used as the studied cells. HE staining was used to observe the histomorphology and observed by Terminal deoxynucleotidy Transferase- mediated d UTP nick end labeling（ TUNEL） and computer image analysis system for the expression of apoptosis rate. Results： The apoptosis rate in the Supplemting Ying- Wei Formula groups were decreased significantly and compared with the aged group there was statistical significance（ P 0. 05）. Between three dose groups,middle dose group had obvious differences from the other groups and there was statistical significance（ P 0. 01）. The low and high dose groups had no significant difference（ P 0. 05）. Conclusion： Supplemting Ying- Wei Formula can decrease the apoptosis rate of skin epidermis aging so as to delay the skin aging.