摘要
目的了解13株卡介苗(bacille calmette-guérin,BCG)基因组中由PE/PPE基因家族编码的B细胞抗原表位分布情况,分析其对BCG免疫保护力的潜在影响。方法从国际免疫表位数据库(Immune Epitope Data base,IEDB)中检索结核分枝杆菌B细胞抗原表位,与结核分枝杆菌参考菌株H37Rv的蛋白质组进行序列比对,确定PE/PPE基因家族编码B细胞抗原表位序列;并与13株BCG基因组进行序列比对,提取表位编码序列,分析其在BCG中的分布与变异。结果 6个PE/PPE基因家族的基因编码28个B细胞抗原表位,21个B细胞抗原表位在13株BCG中高度保守,7个B细胞抗原表位在BCG中发生基因变异,变异表位分别产生于BCG的不同形成期。结论 PE/PPE基因家族所表达的蛋白大多为细菌表面蛋白,PE/PPE蛋白家族中B细胞抗原表位的变化可能对BCG的保护力产生影响,应继续开展基于B细胞表位的BCG遗传特征研究。
Objective To understand the distribution of B cell epitopes coded with PE/PPE gene family in 13 BCG strains and the influence on BCG immunity. Methods Mycobacterium tuberculosis B cell Epitopes was retrieved from Immune Epitope Data for the alignment with sequence of B cell epitopes of proteome of M. tuberculosis strain H37 Rv to determine the PE/PPE gene family coding B cell epitopes. Then the alignment between DNA sequence coding epitopes and genomes of 13 BCG strains was conducted,and the coding sequence in BCG genomes was extracted to analyze its distribution and mutation in BCG. Results Six PE/PPE genes encoded 28 B cell epitopes. 21 B cell epitopes were highly conservative in 13 BCG strains,7 B cell epitopes showed gene mutation in the BCG genomes. All gene variations in B cell epitopes could occur in different formation periods of BCG. Conclusion PE/PPE proteins expressed by the PE/PPE gene family mostly are bacterial surface protein. The gene mutation of B cell epitope in PE/PPE protein family may affect the BCG protection. It should continue to carry out research on BCG genetic characteristics based on B cell epitopes.
出处
《疾病监测》
CAS
2016年第4期282-287,共6页
Disease Surveillance
基金
国家科技重大专项(No.2013ZX10003006-002-001)
