基于SELDI-TOF-MS技术的奶牛乳热血浆蛋白质组学分析

Plasma Proteomics Analysis of Dairy Cows with Milk Fever Using SELDI-TOF-MS

奶牛乳热是围产期奶牛营养代谢性疾病,对于奶牛乳热在病理方面少有血浆蛋白质组学的研究。本研究目的是探究奶牛乳热在蛋白质组学层面的变化。试验21头患有奶牛乳热的奶牛和59头健康奶牛作为试验动物。试验通过SELDI-TOF-MS技术检验得到24个差异肽段的峰值,其中10个肽段经过Swissport Protein数据库搜索鉴定为10种差异蛋白。结果相对于对照组,乳热组中表达上调的蛋白4个;表达下调的蛋白2个;还有既表达上调又表达下调的蛋白4个。结论本次试验应用SELDI-TOF-MS技术鉴定了几个在奶牛发生乳热时表达的差异蛋白质。这些发现也为以后揭示奶牛乳热代谢性改变提供了依据。

Milk fever is an important metabolic disorder of dairy cows at calving. There have been many studies on the patho-physiology of milk fever,but the plasma proteomics of milk fever has not been reported.The aim of this study was to investigate nov-el changes in the plasma proteomics of cows with milk fever. Plasma samples were obtained from 21 Holstein cows with milk fever（T）,and 59 control Holstein cows without milk fever（C）.24 differential peptide peaks in the plasma of T and C cows were isolat-ed by SELDI-TOF-MS. 10 of these peaks were identified by Swissport Protein Database. The petide peaks represented 10 uniqueproteins, and had significant alterations in peaks as determined. The up-regulated proteins were 4,and the down-regulated pro-teins were 2. The other proteins which were up- regulated as well as down- regulated were 4. In conclusion,we were able touse SELDI-TOF-MS to identify several novel plasma proteins in cows with milk fever. These findings may reveal new metaboli-techanges in cows with milk fever.