异育银鲫嗜水气单胞菌的分离鉴定与病理组织学观察

Isolation and Identification of Aeromonas hydrophlia and histopathological examination

为鉴定吉林省某养殖场所养殖异育银鲫(carassius auratus gibelio)死亡原因,在濒死鱼的肝、胰、脾、肾脏分离得到一株优势菌,经细菌形态、常规理化特性鉴定、人工感染试验、16S r RNA基因序列测定、病理解剖学及病理组织学观察,结果表明:分离株为气单胞菌属的嗜水气单胞菌,并将其命名为JL-PW-3。该分离株对环丙沙星、氟苯尼考、强力霉素、氯霉素等高度敏感;对庆大霉素、阿米卡星、阿莫西林、阿奇霉素、红霉素、恩诺沙星、磺胺间甲氧嘧啶等完全耐药。

In order to identify the cause of death of carassius auratus gibelio in farms in Jilin Province,a dominant bacteriumwas isolated from liver,hepatopancreas,spleen and kidney in dying fish.All the results of morphological observation,physical andbiochemical identification assays,artificial infection test,determination of 16 S r RNA gene sequences and pathological and histolog-ical observation conformed that this bacterium was Aeromonas hydrophlia,named as JL-PW-1.This isolate was highly sensitive tociprofloxacin,florfenicol,doxycycline,and chloramphenicol,but completely resistant to gentamicin,amikacin,amoxicillin,azithro-mycin,erythromycin,enrof- loxacin,sulfamono methoxine and other drugs.