前列腺癌和前列腺增生中α辅肌动蛋白-1的表达及其临床意义

The expression and clinical significance of α-actinin-1 in prostate cancer and benign prostatic hyperplasia

目的探讨α辅肌动蛋白-1(α-actinin-1,ACTN1)在前列腺癌(PCa)和前列腺增生(BPH)中的表达及临床意义。方法收集2007年1月—2014年10月我校附属医院收治的PCa和BPH患者资料,按一定的入组标准筛选,采用免疫组织化学法检测30例PCa组织和30例BPH组织中ACTN1的表达情况,采用蛋白免疫印迹(Westernblot)方法检测其中的18例PCa组织和20例BPH组织中ACTN1的相对表达量。结果免疫组织化学结果显示,ACTN1在PCa和BPH两组中的阳性表达率分别为76.7%和20.0%,差异有统计学意义(P<0.01);Western blot结果显示,ACTN1在PCa组中的相对表达量(0.591±0.182)明显高于BPH组(0.037±0.052),差异有统计学意义(P<0.05);免疫组织化学结果中,ACTN1在PCa中的表达在不同年龄、血清前列腺特异抗原(PSA)水平、是否有骨转移、是否有淋巴结转移患者之间差异无统计学意义(P>0.05),而在不同Gleason分级、T分期患者之间差异有统计学意义(P<0.05)。结论 ACTN1在PCa组织中的表达明显高于BPH组织,且在PCa组织中的表达与Gleason分级、T分期之间可能存在关联。

Objective To investigate the expression and clinical significance of α-actinin-1 protein (ACTN1) in prostate cancer (PCa) and prostatic hyperplasia (BPH). Methods The clinical data of patients with PCa or BPH treated in our school affiliated hospital were collected between January 2007-October 2014, according to certain criteria. Immunohistochemistry method was used to detect the expression of ACTN1 in 30 samples of PCa and 30 samples of BPH tissues. Western blot assay was used to detect the relative expression of ACTN1 in 18 samples of PCa and 20 samples of BPH tissues in two groups. Results The result of immunohistochemistry showed that the positive expression rates of ACTN1 were 76.7%and 20%in PCa and BPH groups respectively. The difference was statistically significant (P<0.01). Western blot assay showed that the relative expression of ACTN1was significantly higher in PCa group (0.591±0.182) than that in BPH group (0.037 ± 0.052, P < 0.05). There was no significant difference in expression level of ACTN1 between&nbsp;different age groups. There was no significant difference in serum prostate specific antigen (PSA) level between patients with or without bone metastasis, and patients with or without lymph node metastasis (P > 0.05). There were significant differences in ACTN1 levels between different Gleason score and T staging groups (P<0.05). Conclusion The expression ofα-actinin-1 is significantly higher in PCa tissues than that in BPH tissues. There is the relationship between expression of ACTN1, Gleason scores and T staging.