摘要
The whitefly Bemisia tabaci is a serious threat in tomato cultivation worldwide as all varieties grown today are highly susceptible to this devastating herbivorous insect.Many accessions of the tomato wild relative Solanum pennellii show a high resistance towards B. tabaci. A mapping approach was used to elucidate the genetic background of whiteflyresistance related traits and associated biochemical traits in this species. Minor quantitative trait loci（QTLs） for whitefly adult survival（AS） and oviposition rate（OR） were identified and some were confirmed in an F2BC1 population, where they showed increased percentages of explained variance（more than 30%）. Bulked segregant analyses on pools of whiteflyresistant and-susceptible F2 plants enabled the identification of metabolites that correlate either with resistance or susceptibility. Genetic mapping of these metabolites showed that a large number of them co-localize with whiteflyresistance QTLs. Some of these whitefly-resistance QTLs are hotspots for metabolite QTLs. Although a large number of metabolite QTLs correlated to whitefly resistance or susceptibility, most of them are yet unknown compounds and further studies are needed to identify the metabolic pathways and genes involved. The results indicate a direct genetic correlation between biochemical-based resistance characteristics and reduced whitefly incidence in S. pennellii.
The whitefly Bemisia tabaci is a serious threat in tomato cultivation worldwide as all varieties grown today are highly susceptible to this devastating herbivorous insect.Many accessions of the tomato wild relative Solanum pennellii show a high resistance towards B. tabaci. A mapping approach was used to elucidate the genetic background of whiteflyresistance related traits and associated biochemical traits in this species. Minor quantitative trait loci（QTLs） for whitefly adult survival（AS） and oviposition rate（OR） were identified and some were confirmed in an F2BC1 population, where they showed increased percentages of explained variance（more than 30%）. Bulked segregant analyses on pools of whiteflyresistant and-susceptible F2 plants enabled the identification of metabolites that correlate either with resistance or susceptibility. Genetic mapping of these metabolites showed that a large number of them co-localize with whiteflyresistance QTLs. Some of these whitefly-resistance QTLs are hotspots for metabolite QTLs. Although a large number of metabolite QTLs correlated to whitefly resistance or susceptibility, most of them are yet unknown compounds and further studies are needed to identify the metabolic pathways and genes involved. The results indicate a direct genetic correlation between biochemical-based resistance characteristics and reduced whitefly incidence in S. pennellii.
基金
financially supported by the Technical Top Institute of Green Genetics(TTI-GG
Resistance mechanisms against whitefly in tomato project:3360124600),Monsanto Vegetable Seeds(Bergschenhoek,The Netherlands),Nunhems NL(Nunhem,the Netherlands),and Wageningen University and Research Centre
partially funded by the Netherlands Metabolomics Centre and the Centre of Biosystems Genomics,which are both part of the Netherlands Genomics Initiative/Netherlands Organization for Scientific Research
