从HBsAg阳性携带者血清中提取HBcAg用于固相放免法检测抗HBc

ISOLATION OF HBcAg FROM PLASMA OF ASYMPTOMATIC HBsAg CARRIERS FOR USE IN MICRO-SPRIA TO DETECT ANTI-HBc

1971年Almeida等把血清中Dane颗粒用tween-80裂解得到直径为27μm的HBcAg,并指出它不同于HBsAg。此后,国外先后从乙肝患者的肝细胞或血浆中提到粗制或精制的HBcAg。1978年本研究室与解放军302医院协作应用密度梯度超速离心法从尸肝中精制了HBcAg,并建立了国家标准品。HBcAg的提取对研究乙肝病毒,及乙肝的临床和实验室研究具有重要意义,其实用价值之一是可用于检测抗HBc。

A method has been developed to extract HBcAg from plasma of asympotomatic HBsAg carriers rich in Dane's particles. HBcAg was prepared from the plasma by precipitating the specific immune complex and treating it with NP-40. After standardization, under required conditions, the HBcAg could be used in MICRO-SPRIA to detect anti-HBc. This assay has been found to be specific, sensitive and stable to a certain extent. A simple way of getting HBcAg necessary for use in hepatitis laboratories is provided with our method.