红景天苷对高原红细胞增多症大鼠防治作用的实验研究

Experimental Study of Preventive Effect of Salidroside on Mice with High Altitude Polycythemia

目的:观察红景天苷对高原红细胞增多症大鼠的防治作用。方法:实验大鼠随机分为空白组、模型组、红景天苷高、中、低剂量组和阳性药组,测定红细胞数、血红蛋白含量、红细胞压积,全血黏度、血浆黏度,采用酶联免疫法测定一氧化氮(NO)、一氧化氮酶(NOS)、内皮素(ET)-1、血管内皮生长因子(VEGF)。结果:模型组大鼠红细胞(RBC)、血红蛋白(Hb)、血球压积(HCT)与空白组比较,显著升高(P<0.05)。与模型组比较,红景天苷高、中、低剂量具有降低上述指标的作用,尤其是中、高剂量组作用显著(P<0.05)。模型组大鼠血浆黏度、全血黏度与空白组比较,显著升高(P<0.05)。与模型组比较,红景天苷高、中、低剂量具有降低全血黏度、血浆黏度的作用,尤其是高剂量作用显著(P<0.05)。模型组大鼠NO、NOS与空白组比较显著降低(P<0.05),ET-1、VEGF显著升高(P<0.05)。与模型组比较,红景天苷高剂量具有显著升高NO、NOS,降低VEGF的作用(P<0.05);红景天苷高、中剂量具有显著降低ET-1的作用(P<0.05)。结论:红景天苷对高原红细胞增多症大鼠有较好的防治作用。

Objective：To observe the preventive effect of salidroside on mice with high altitude polycythemia. Methods：The experimental mice were divided into blank group,model group,high,mid and low dose group of salidroside,positive drug group. Mesured red blood count,hemoglobin content,hematocrit,whole blood viscosity and plasma viscosity,and detected NO,nitric oxide synthase（NOS）,endothelin（ET）- 1,vascular endothelial growth factor（VEGF） by the method of enzyme- linked immunoassay. Results：Comparing with blank group,Red Blood Cell（RBC）,hemoglobin（Hb） and hematocrit（HCT） of mice in model group were all increased significantly（P〈0.05）. Comparing with model group,above indexes of high,mid and low dose group of salidroside were decreased,especially,those improvement of mid and low dose group being significant（P〈0.05）.Comparing with blank group,plasma viscosity and whole blood viscosity of mice in model group were increased significantly（P〈0.05）. Comparing with model group,whole blood viscosity and plasma viscosity of high,mid and low dose group of salidroside were decreased,especially,those improvement of high dose group being significant（P〈0.05）. Comparing with blank group,NO and NOS of mice in model group were all decreased significantly（P〈0.05）,ET- 1 and VEGF were increased significantly（P〈0.05）. Comparing with model group, high dose of salidroside had effect on increasing NO and NOS and decreasing VEGF significantly（P〈0.05）;high and mid dose of salidroside had effect on decreasing ET- 1（P〈0.05）. Conslusion：Salidroside has good preventive effect on mice with high altitude polycythemia.