摘要
A laboratory-scale intermittent aeration bioreactor was investigated to treat biologically pretreated coal gasification wastewater that was mainly composed of NH_3-N and phenol.The results showed that increasing phenol loading had an adverse effect on NH_3-N removal;the concentration in effluent at phenol loading of 40 mg phenol/（L·day） was 7.3 mg/L, 36.3%of that at 200 mg phenol/（L·day）. The enzyme ammonia monooxygenase showed more sensitivity than hydroxylamine oxidoreductase to the inhibitory effect of phenol, with32.2% and 10.5% activity inhibition, respectively at 200 mg phenol/（L·day）. Owing to intermittent aeration conditions, nitritation-type nitrification and simultaneous nitrification and denitrification（SND） were observed, giving a maximum SND efficiency of 30.5%.Additionally, ammonia oxidizing bacteria（AOB） and denitrifying bacteria were the main group identified by fluorescent in situ hybridization. However, their relative abundance represented opposite variations as phenol loading increased, ranging from 30.1% to 17.5%and 7.6% to 18.2% for AOB and denitrifying bacteria, respectively.
A laboratory-scale intermittent aeration bioreactor was investigated to treat biologically pretreated coal gasification wastewater that was mainly composed of NH_3-N and phenol.The results showed that increasing phenol loading had an adverse effect on NH_3-N removal;the concentration in effluent at phenol loading of 40 mg phenol/（L·day） was 7.3 mg/L, 36.3%of that at 200 mg phenol/（L·day）. The enzyme ammonia monooxygenase showed more sensitivity than hydroxylamine oxidoreductase to the inhibitory effect of phenol, with32.2% and 10.5% activity inhibition, respectively at 200 mg phenol/（L·day）. Owing to intermittent aeration conditions, nitritation-type nitrification and simultaneous nitrification and denitrification（SND） were observed, giving a maximum SND efficiency of 30.5%.Additionally, ammonia oxidizing bacteria（AOB） and denitrifying bacteria were the main group identified by fluorescent in situ hybridization. However, their relative abundance represented opposite variations as phenol loading increased, ranging from 30.1% to 17.5%and 7.6% to 18.2% for AOB and denitrifying bacteria, respectively.
基金
supported by State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (No. 2015DX02)
