miR-204对乳腺癌MCF-7细胞增殖及凋亡的影响

Effect of miR-204 on proliferation and apoptosis of breast cancer cell line MCF-7

目的探讨miR-204在人乳腺癌组织及MCF-7细胞中的表达水平及其对乳腺癌细胞增殖及凋亡的影响。方法提取癌症和肿瘤基因组图谱(the cancer genome atlas,TCGA)数据库中浸润性乳腺癌的miR-204相关数据进行汇总,转染miR-204过表达病毒,筛选稳定转染细胞株并通过RT-q PCR检测转染率,MTT法检测过表达miR-204后乳腺癌MCF-7细胞的增殖情况,流式细胞仪检测细胞凋亡情况。结果 miR-204在浸润性乳腺癌组织中的表达水平较癌旁组织明显下调(P<0.001),且与淋巴结转移和远处转移相关(P<0.05)。过表达miR-204能够抑制乳腺癌MCF-7细胞的增殖能力(P<0.05);miR-204上调后,细胞凋亡率达到(34.7±1.9)%,细胞凋亡能力明显增强(P<0.001)。结论 miR-204可抑制乳腺癌细胞增殖并介导细胞凋亡。

Objective To explore the expression of miR-204 in human breast cancer tissues and the MCF-7 cell line ,and to investigate the influence of miR-204 on proliferation and apoptosis of breast cancer cells. ：Methods The miR-204 sequence was cloned based on information in the TCGA database, then it was used to generate recombinant virus and than transfected into MCF-7 cell,and the expressed microRNA was detected in infected cells using RT-qPCR. The proliferative ability of infected cells expressing miR-204 was tested using the MTF assay. Flow cytometry analysis was used to determine extent of apoptosis. Results Expression of miR-204 was significantly lower in invasive breast cancer tissues than in normal breast tissue;expression correlated with lymph node and cancer metastasis. MTT assays showed that overexpression of miR-204 inhibited MCF-7 cell proliferation, and flow cytometry suggested that it significantly increased the rate of apoptosis. Conclusion miR-204 can inhibit breast cancer cell proliferation and induce apoptosis.