GP73在肝细胞癌诊断中的应用价值

Diagnostic potential of GP73 for hepatocellular carcinoma

目的探讨高尔基蛋白73(Golgi protein-73,GP73)在肝细胞癌(heptocellular carcinoma,HCC)患者血清中的表达及其对HCC的诊断价值。方法收集人外周血清62例,其中HCC 31例(HCC组),健康志愿者31名(健康对照组)。采用Western blot法检测两组血清中GP73的表达,制作受试者工作特征曲线(receiver operating characteristic,ROC),以曲线下面积(area under curve,AUC)评价GP73诊断HCC的效能。结果 HCC组和健康对照组血清中GP73阳性表达率分别为74.19%(23/31)和9.68%(3/31),两组比较差异有统计学意义(P<0.01)。当设定GP73浓度82.2 ng/m L为诊断临界值时,ROC曲线下面积为0.823,其检测HCC的敏感度和特异度均达到最优,分别为90%和78%。结论 GP73诊断HCC具有较高的敏感度和特异度,是较好的HCC诊断标志物。

Objective The aim of this study was to detect GP73 in the serum of healthy individuals and patients with hepatocellular carcinoma （HCC） using Western blotting and to assess the diagnostic sensitivity and specificity of the protein. Methods Expression of GP73 was measured in healthy and HCC individuals using Western blotting. The diagnostic performance of GP73 was evaluated using a receiver operating characteristic （ROC） curve. Results GP73 protein was expressed at significantly higher levels in HCC patients than in healthy individuals （P〈0.001）. Using a GP73 concentration of 82.2 ng/mL as the diagnostic threshold for HCC, the area under the ROC curve was 0.823, sensitivity was 90% and specificity was 78%. Conclusion GP73 may be a reliable serum marker for diagnosis of HCC.