护骨胶囊对去卵巢大鼠血清骨代谢指标的影响

Effect of Hugu capsules on bone metabolic indexes in ovariectomized rats

目的观察去卵巢大鼠血清骨代谢指标的改变及护骨胶囊(HG)的调节作用。方法采用去卵巢(OVX)模型,分假手术(Sham),去卵巢(OVX),仙灵骨葆(Pos)和护骨胶囊低、中、高剂量组(HG-L、HG-M和HG-H)。术后2d连续灌胃给药3个月,取血清检测骨代谢指标。结果1与Sham组比较,OVX组骨形成指标ALP、BALP、UcOC、PINP和骨吸收指标CTX均显著升高,但TRAP5a,TRAPb水平降低;OPG和OPG/RANKL比值显著降低。2与OVX组比较,HG-L、H组进一步升高ALP,HGH组也升高BALP,HG-L、HG-M组降低BALP;Pos组降低ALP和BALP水平。HG和阳性药物给药组降低了UcOC和PINP水平,同时也降低CTX水平。HG-L组的TRAP5a升高;HG-L、HG-M和Pos组使TRAP5b升高。OPG在HG-H和Pos组增加;OPG/RANKL比值HG和阳性药物给药组均升高。结论OVX使部分骨形成和骨吸收指标增加,表现为骨高转换,HG和仙灵骨葆都能降低这些指标。由于HG能在OVX基础上进一步升高ALP,HG-H也升高BALP,而仙灵骨葆没有进一步提高ALP和BALP水平,提示护骨胶囊刺激成骨的作用较强。

Objective To study the change of bone metabolic indexes in ovariectomized（ OVX） rats and the regulatory effect of the Hugu capsules（ HG）. Methods Rats were divided into Sham group,OVX group,Xianlinggubao group（ Pos）,and low,medium,and high dose HG group（ HG-L,HG-M,and HG-H）. The rats were administrated medicine intragastricly for 3 months.The serum bone metabolic indexes were detected. Results（ 1） ALP,BALP,UcOC,PINP,and CTX increased,but the levels of OPG,TRAP5 a,b,and OPG /RANKL ratio decreased significantly in OVX group compared to those in Sham group.（ 2）Compared to those in OVX group,ALP increased in all HG groups. BALP increased in HG-H,but decreased in HG-L and HG-M group. ALP and BALP decreased Pos group. Levels of UcOC,PINP,and CTX decreased in HG groups and Pos group. The level of TRAP5 a increased in HG-L group. TRAP5 b increased in HG-L,HG-M,and Pos group. OPG levels increased in HG-H and Pos group. The OPG /RANKL ratios increased in all the medicine groups. Conclusion OVX increases part of the bone metabolic markers and shows a high bone turnover. HG inhibits high bone turnover. HG further increases ALP and HG-H increases BALP,while Xianlinggubao does not increase ALP and BALP levels,indicating that the stimulation effect of bone formation is better by HG than that by Xianlinggubao.