猴头菌菌丝体萜类物质提取优化及抗氧化研究

Extracting technology and antioxidant activity of terpenoids substances from Hericium erinaceum

为了提高猴头菌菌丝体中萜类物质提取率,通过酶解和酸解关键技术对猴头菌菌丝体进行前处理,再通过溶剂回流提取方法进行工艺参数优化;为了研究猴头菌菌丝体中萜类物质的抗氧化性能,采用体外抗氧化活性法测定萜类物质的超氧阴离子、DPPH·和ABTS+·自由基清除能力.实验结果表明:酶解和酸解相结合的前处理,够显著提高猴头菌菌丝体中萜类物质的提取率;通过单因素试验和响应面分析法(RSM)实验,得到优化的回流提取工艺条件为料液比1∶36g/mL,提取温度63℃,提取时间92 min,猴头菌菌丝体中萜类小分子活性物质的提取率从1.80%提高到3.39%;萜类物质清除超氧阴离子的半数效应质量浓度EC50=0.926mg/mL;清除DPPH·自由基的半数效应质量浓度EC50=1.206mg/mL;清除ABTS+·自由基的半数效应质量浓度EC50=0.403mg/mL.该研究可为猴头菌菌丝体中萜类物质的工业提取和应用提供新的参考.

In order to increase the yield of terpenoids substances from hericium erinaceum,we developed a new method for the extraction of terpenoids substances from Hericium erinaceum with fermentation which was pretreated with enzymatic and acid hydrolysis and then optimized by the method of ethyl alcohol reflux extraction through mono-factorial experiment and response surface methodology（RSM）.The antioxidant activities were measured by the superoxide action for the scavenging capacity of both DPPH· and ABTS＋· radicals.The pretreatment with enzymatic and acid hydrolysis can significantly help increase the extraction yield of terpenoids substances from Hericium erinaceum mycelia material.The optimum process parameters for extraction process were obtained：ethanol concent ration 65%,liquid material ratio 1∶36g/mL,extracting temperature 63 ℃ and extracting time 92 min.The yield of substances from hericium erinaceum was increased to 3.39% from 1.80%.The terpenoids substances showed significant antioxidant activities.The EC50 of superoxide action,DPPH· and ABTS＋· were 0.926 mg/mL,1.206mg/mL and 0.403mg/mL,respectively.This work can provide a new perspective on the extraction of terpenoids from Hericium erinaceum mycelia material and also has a potential industrial application.