NOD小鼠调节性T细胞的功能表型分析

Analysis on the function and phenotype of regulatory T cells in non-obese diabetic mice

目的:比较和探讨NOD(Non-Obese Diabetic)小鼠与正常BALB/C小鼠脾脏中CD4^+CD25^+调节性T细胞(regulatory T cell,Treg)的功能表型分子、抑制性细胞因子的表达差异及意义。方法:采用流式细胞检测技术,测定NOD小鼠(实验组)和正常BALB/C小鼠(对照组)脾脏中Treg细胞(CD4^+CD25^+Foxp3^+)的比例、功能表型分子(CTLA-4、GITR)和抑制性细胞因子(IL-10、TGF-β)的表达比例和平均荧光强度(mean fluorescence intensity,MFI)。结果:NOD小鼠脾脏中CD4^+CD25^+Foxp3^+Treg细胞比例与对照组相比无显著差异,但Foxp3^+的MFI显著降低;NOD小鼠Treg细胞表达CTLA-4、GITR、IL-10、TGF-β均发生不同程度降低。结论:NOD小鼠Treg细胞功能表型分子表达下降,提示其免疫抑制功能降低,这可能与Ⅰ型糖尿病(typeⅠdiabetes mellitus,T1DM)的发病有一定关系。

Objective： To compare the expression of surface functional molecules and immunosuppressive cytokines（ CD4~＋CD25~＋） of regulatory T cells（ Tregs） in non-obese diabetic（ NOD） mice and BALB /C mice. Methods： The percentage and expression intensity of key transcription factor Foxp3~＋,surface molecules（ CTLA-4,GITR） and cytokines（ IL-10 and TGF-β） of CD4~＋CD25~＋Tregs in spleen of mice were determined with fluorescent antibody staining and analyzed by flow cytometry. Results： There was no significant difference in the percentage of CD4~＋CD25~＋Foxp3~＋Tregs between NOD and control mice. However,CD4~＋CD25~＋Tregs in NOD mice had led to significantly reduced expression levels of Foxp3~＋,surface molecules（ CTLA-4,GITR） and cytokines（ IL-10 and TGF-β）,when expressed as the mean fluorescence intensity（ MFI） respectively. Conclusion： Our findings confirmed impaired function of CD4~＋CD25~＋Tregs in NOD mice that may facilitate the pathogenesis of T1 DM.