甜菜不育系育性核基因差异对应的2DE蛋白图谱差异分析

The variance analysis between nuclear gene and two-dimensional protein electrophoresis about sterile lines of sugar beet

通过对内蒙古农牧业科学院特色作物所提供的选育过程中的不育系及对应保持系和选育稳定的不育系及对应保持系进行田间花粉调查分类,并对受试材料进行VNTR微卫星分子标记鉴定,从不育系中选出拥有Owen型不育胞质,然后对不同出粉情况的不育系进行二年生苗期、花期2DE双向蛋白电泳,并进行蛋白图谱差异分析,得出苗期在选定的参考组内385个蛋白点图谱中发现差异点12个,在参考组内的花期比对发现蛋白差异点总和23个,并且包含前者发现的12个蛋白差异点;后者中编号3~9各不育系同参考不育系对比发现差异点数分别为7,5,5,6,8,11,18个;参考组间的差异蛋白点数为23个,在各个差异中所共存的差异蛋白点数为4个,其中对拥有Owen型不育胞质且出粉情况较高的不育植株即编号9进行差异蛋白分析,发现5个不被参考组差异所覆盖的蛋白点,初步推测核质互作的核基因并没有抑制不育质基因的编码表达,而是核质基因共同表达后的蛋白系列调控导致甜菜育性恢复。

This paper m ainly focused on the tw o-dim ensional protein electrophoresis difference betw een various cytoplasmicmale sterility （CM S） about suger beets supplied by Inner Mongolia academ y of agricultural and anim al husbandry sciences.Seven kinds of suger beet had been selected through pollen research and m ark er-assisted selection.According to VNTRm icrosatellite m olecular m arker identification method the suger beets had the Owen infertility cytoplasm.Then through theflorescence analysis of reference group and treatm ent group between pollen situation and two -dim ensional proteinelectrophoresis, 23 difference protein spot had been found including 12 difference protein spot found in seedling stage ofreference group.After further analysis of the tw o-dim ensional protein electrophoresis of CMS between reference group andtreatm ent g roup,the difference protein spot num ber were 7 , 5 , 5 , 6 , 8 , 1 1 , 18.In the same time the 9 type with Owen infertilitycytoplasm and good pollen situation was analyzed and 5 difference protein spot had not been included in reference group.Prelim inary speculation was that nuclear genedid not control infertility cytoplasm gene protein expression but through proteinregulator control system lead to fertility restoring.