四角蛤蜊酶解多肽抗氧化活性及稳定性研究

Study on the Antioxidative Activity and Stability of Mactra Veneriformis Enzymolysis Polypeptide

在研究四角蛤蜊酶解多肽羟基自由基清除能力和DPPH自由基清除的前提下,以DPPH自由基清除能力为主要指标,探究酶解多肽在温度变化、pH变化、反复冻融、紫外干预及常见金属离子干预等条件下的抗氧化活性稳定性。结果表明,四角蛤蜊酶解多肽具有较强的抗氧化活性。在高温、酸性、紫外干预、冻融多种常见金属离子(Mg2+、Na+、K+、Fe2+)等条件下,酶解多肽的抗氧化活性被保存得较好;在碱性、Cu2+、Zn2+、Ca2+等条件下,酶解多肽的抗氧化活性被破坏程度较大。

Under the premise of studying on the scavenging activity of mactra veneriformis enzymolysis polypeptide on hydroxyl radical and DPPH radical, with the scavenging activity of enzymolysis polypeptide on DPPH radical as the main index, this paper explored the antioxidant activity stability of enzymolysis polypeptide by temperature, pH, repeated freeze-thaw, UV intervention and common metal ions. The results showed that mactra veneriformis enzymolysis polypeptide has strong antioxidant activity. Under the conditions of high temperature, acid, UV intervention, freezing thawing and many kinds of common metal ion （ Mg^2 ＋ , Na^＋ , K^ ＋ , Fe^2 ＋ ）, the antioxidant activity of enzymolysis polypeptide was well preserved. Under the condition of alkaline, Cu^2 ＋ , Zn^2 ＋ and Ca^2 ＋ , the antioxidant activity of enzymolysis polypeptide was destroyed.