替加环素联合亚胺培南对多重耐药鲍曼不动杆菌的体外抗菌活性研究

In Vitro Activity of Tigecycline Combined with Imipenem against Multi drug- Resistant Acinetobacter Baumann

目的探讨替加环素(tigecyclin)联合亚胺培南对多重耐药鲍曼不动杆菌体外抗菌的效果,探索多药联合应用对多重耐药鲍曼不动杆菌的临床用药新途径。方法收集临床分离的40株多重耐药鲍曼不动杆菌,采用微量肉汤稀释法、棋盘法分别检测替加环素、亚胺培南单独及联合使用的最低抑菌浓度(MIC),计算药物联合使用抑菌浓度(FIC)指数,利用FIC指数判定替加环素和亚胺培南联用时对多重耐药鲍曼不动杆菌体外抗菌的效果。结果替加环素联合亚胺培南能显著降低各自的MIC值,其中表现为协同作用的菌株占70.00%,表现为相加作用的菌株占25.00%,发生无关作用的占5.00%,无拮抗作用发生。结论替加环素联合亚胺培南可对多重耐药鲍曼不动杆菌的感染进行治疗。

Objective To investigate the in vitro activity of tigecycline combined with imipenem against multi drug- resistant Acinetobacter baumann,and to explore the new way of clinical medicine administration against multi drug- resistant Acinetobacter baumann. Methods Agar dilution method and checkerboard method were used for the MIC testing of tigecycline combined with imipenem against 40 multi drug- resistant Acinetobacter baumannii strains,and MIC value was used to calculate the fractional inhibitory concentration（ FIC） index. Results The MIC of tigecycline and imipenem were reduced significantly when using by tigecycline combined with imipenem. The FIC results showed that the main interaction was synergism（ 70. 00%） and additivity（ 25. 00%）,with only 5. 00% of inefficiency. No antagonism was observed. Conclusion Tigecycline combined with imipenem can be used to treat the infection of multi drug- resistant Acinetobacter baumann.