近缘两个木薯品种块根蛋白质组比较分析

Comparative Analysis of Proteomics in Tuberous Roots between Two Kindred Cassava Varieties

以木薯(Manihot esculenta Crantz)栽培种华南9号(SC9)及其近缘野生种W14的块根为研究对象,利用蛋白质组学技术,分析了这两个品种块根的蛋白质差异情况,并对差异蛋白质的功能进行了分类。结果表明:主要有22个蛋白质出现较大的差异表达,其中16个为已知功能的蛋白质,可分为7类,包括信号传导、核酸代谢和碳与能量代谢等。其中,与碳和能量代谢相关的蛋白质果胶酶,以及与光合作用相关的苹果酸合成酶在SC9块根细胞中的表达量显著高于在W14中的。进一步的RT-PCR定量分析发现PME3基因在SC9块根中的表达量显著高于在W14中的。

Using cultivar species（ Manihot esculenta Crantz CV. South China 9,SC9） and wild species（ Manihot esculenta subsp. flabellifolia,W14） as material to analyze their differences by comparative proteomics. The results showed that there were22 proteins,which should be clustered seven types among 16 proteins that function was known,including signal transfer,RNA or DNA synthesis,carbon or energy metabolism,photosynthesis,chaperones,anti-oxidation and proteins synthesis. The expression level of Pectinesterase 3（ PME3） and Malate synthase were significant higher in the root cells of SC9 than that of in W14. The relative expression level of PME3 in the root cells of SC9 was higher than that of in W14 by RT-PCR quantitative analysis.