产纤维素酶地衣芽孢杆菌B.LY02摇瓶发酵条件优化

Optimization of Shake Flask-ferment Conditions for Bacillus Lichenniformis B. LY02 Producing Cellulase

为了提高产纤维素酶地衣芽孢杆菌（Bacillus licheniformis LY02,B.LY02）的产酶能力,采用单因素试验对该菌株产酶的摇瓶发酵条件进行了优化。优化结果显示：菌株B.LY02发酵培养基的最适碳源为麦芽糖,氮源为酵母膏,初始pH值为5.0,培养温度为37℃,培养时间为30 h,装液量为60 mL（250 m L三角瓶）,接种量（体积分数）为2%,摇床转速180～200 r/min。通过优化发酵条件,菌株B.LY02的产酶活性达到了0.683 5 U/mL,比优化前提高了约27.73%。

In order to improve the production of cellulase from Bacillus lichenniformis LY02( B. LY02) strain,the shake flask-ferment conditions of cellulase produced by B. LY02 were optimized by using single factor experiments. The results show that the optimal carbon source is maltose and the optimal nitrogen source is yeast extract in B. LY02 fermentation medium. While the initial p H,cultivation temperature value,incubation time of B. LY02 fermentation medium is 5. 0,37 ℃ and 30 h respectively. Besides,loading medium volume is 60 m L( 250 m L triangular flask),initial inoculum is 2%,and the shaking speed is 180 r/min to 200 r/min. Under the optimal fermentation condition,the cellulase activity of B. LY02 was up to 0. 683 5 U / m L,which is increased by about 27. 73% than the original culture conditions.