灰树花多糖D组分对细颗粒物诱导的人支气管上皮细胞损伤的保护作用

Protective effects of Grifola frondosa D fraction on human bronchial epithelial cells damage induced by fine particulate matter

目的研究灰树花多糖D组分（D-fraction）对细颗粒物（SRM 2786）诱导的人支气管上皮细胞（16HBE）损伤的保护作用。方法利用细胞增殖实验检测SRM 2786或D-fraction对16HBE细胞增殖的影响,确定给药浓度;将16HBE细胞分7组,分别为对照组、模型组（125μg/m L SRM 2786）和不同浓度D-fraction给药组（分别给予12.5、25、50、100、200μg/m L D-fraction,同时给予125μg/m L SRM 2786）,细胞增殖实验检测细胞存活率,酶联免疫法检测细胞培养液白介素-1β（IL-1β）、白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和粒细胞巨噬细胞刺激因子（GM-CSF）的含量。结果 31.25~500μg/m L SRM 2786处理使细胞存活率显著降低（P〈0.05）。125μg/m L的SRM 2786刺激使16HBE细胞IL-1β、IL-6、TNF-α和GM-CSF释放显著升高（P〈0.05）,D-fraction（≥100μg/m L）显著抑制SRM 2786诱导的炎症细胞因子释放（P〈0.01）,且能显著提高损伤细胞存活率。结论细颗粒物能抑制16HBE细胞增殖、诱导炎症因子释放,D-fraction对细颗粒物造成的细胞损伤有显著保护作用,其作用机制可能与抑制炎症因子释放有关。

Objective To investigate the protective effect of Grifola frondosa D fraction（D-fraction） on human bronchial epithelial（16HBE） cell damage that induced by fine particulate matter（SRM2786）. Methods MTT assay was used to detect the survival rate of 16 HBE cells treated with SRM2786 or D-fraction, and the concentration of SRM2786 or D-fraction used for further experiments was selected. 16 HBE cells were divided into seven groups,including control group, model group（125 μg/m L SRM 2786）, D-fraction group（125 μg/m L SRM 2786＋12.5 or 25 or50 or 100 or 200 μg/m L D-fraction）. MTT assay was used to detect the survival rate, and enzyme-linked immune kits were used to detect the release of interleukin-1β（IL-1β）, interleukin-6（IL-6）, tumor necrosis factor-α（TNF-α） and granulocyte-macrophage colony-stimulating factor（GM-CSF）. Results Compared with the control group, the cell survival rates of 16 HBE cells treated with SRM 2786（31.25-500 μg/m L） were decreased remarkably（P〈0.05）. Treatment with125 μg/m L SRM 2786 could significantly increase the release of IL-1β, IL-6, TNF-α and GM-CSF（P〈0.05）. D-fraction（≥100 μg/m L） could not only significantly improve the survival rate, but also significantly inhibit the release of inflammatory cytokines（P〈0.01）. Conclusion Fine particulate matter can inhibit cell proliferation and induce the release of inflammatory cytokines. D-fraction has a significant protective effect of cell damage caused by fine particulate matter, and the mechanism may be related to inhibit the release of inflammatory factors.