甘遂半夏汤加减甘遂及不同品种甘草对腹水模型大鼠肝脏CYP450m RNA的影响

Comparing the effect of Gansui Banxia decoction plus or reduce kansui and varieties of glycyrrhiza on the CYP450 mRNA of liver of ascites model rats

目的观察含不同品种甘草的甘遂半夏汤加减甘遂甘草反药组合对腹水模型大鼠肝脏CYP450 mRNA的影响。方法采用Walker-256细胞制造癌性腹水模型,将Wistar大鼠按体质量随机分为空白组、模型组、阳性对照组、全方炙甘草组、全方炙光果甘草组、全方炙胀果甘草组、去遂炙甘草组、去遂炙光果甘草组、去遂炙胀果甘草组、去炙甘草组、去炙甘草醋甘遂组,共计11组。空白组和模型组灌胃蒸馏水,其余各给药组灌胃相应药物。给药11天后,摘取肝脏,液氮保存,进行PCR实验。结果在CYP2E1 mRNA表达方面,全方炙甘草组、全方炙胀果甘草表达下调,且优于全方去掉一味或两味反药。三个甘草品种的甘遂半夏汤的CYP3A1 mRNA、CYP3A2 mRNA表达上调,且优于全方去掉一味或两味反药。结论 (1)全方炙甘草组、全方炙胀果甘草组在CYP450表达方面表现出一定的药效作用,其中全方炙甘草组优于全方炙胀果甘草,且均优于全方去掉一味或两味反药组。(2)全方炙光果甘草组则未表现出一定的药效作用,但也未表现出毒性作用,弱于去掉一味或两味反药。

Objective To observe the effect of different varieties of glycyrrhiza and kansui incompatible herbs in Gansui Banxia decoction on liver CYP450 mRNA ascites model rat. Methods The Walker-256 cell was used to manufacture carcinomatous ascites model. The Wistar rats were randomly divided into blank group,model group,positive control group,complete formula with Glycyrrhiza uralensis Fisch.group,complete formula with Glycyrrhiza glabra L. group,complete formula with Glycyrrhiza inflata Bat.group,the Glycyrrhiza uralensis Fisch. Without kansui group,the Glycyrrhiza glabra L. without kansui group,the Glycyrrhiza inflata Bat. without kansui group,prescription without glycyrrhiza group,prescription without glycyrrhiza and kansui group,a total of 11 group. The blank control group and model group were given distilled water,the rest of the drug group were given corresponding drugs. After 11 days of treatment,the liver was removed and stored in liquid nitrogen and saved for PCR experiment. Results The expression of CYP2E1 mRNA in complete formula with Glycyrrhiza uralensis Fisch. group and complete formula with Glycyrrhiza glabra L. Group were down-regulation and superior to add and subtract the prescrip-tion. CYP3A1 and CYP3A2 mRNA expression in the three complete formula groups with three species of glycyrrhiza were up-regulated,and better than add and subtract the prescription. Conclusion（ 1）CYP450 expression in complete formula with Glycyrrhiza uralensis Fisch. group and complete formula Glycyrrhiza inflata Bat. group showed certain effect,which Glycyrrhiza uralensis Fisch. group is better than the roast Glycyrrhiza inflata Bat.,and was better than the group which was added and subtracted the prescription.（ 2） The complete formula with Glycyrrhiza glabra L. group did not show a certain effect,but also did not show toxic effects,the effect was weak than the group which was added and subtracted the prescription.