甘遂半夏汤加减甘遂甘草不同给药次数对腹水大鼠肝脏病理形态的影响

Comparing the effect of Gansui Banxia decoction plus or reduce kansui and glycyrrhiza on pathological morphology of liver of ascites rat under different times

目的同给药次数条件下的甘遂半夏汤加减甘遂甘草反药组合对腹水模型大鼠肝脏病理形态的影响。方法采用Walker-256细胞制造癌性腹水模型,将Wistar大鼠按体重随机分为空白组、模型组、阳性对照组、全方给药一次组、去草给药一次组、去遂给药一次组、去草遂给药一次组、全方给药二次组、去草给药二次组、去遂给药二次组、去草遂给药二次组,共计11组。给药一次组每天上午给药1次,给药二次组上午、下午各给药1次,一二次组每天给药总量一致。腹水长成14天后取材,摘取肝脏,制成病理切片,在光镜下观察大鼠肝脏的病理形态变化,并进行半定量评分。结果模型组肝细胞损伤严重,全方给药一次组及二次组肝损伤较模型组明显减轻,且一次组的减轻程度大于二次组。与全方一次组比较,去草一次组、去遂一次组、去草遂一次组肝损伤加重;与全方二次组比较,去草二次组、去遂二次组、去草遂二次组肝损伤加重。结论全方给药一次组和二次组对肝损伤均有一定的改善作用,给药一次组的改善作用优于给药二次组;全方组损伤修复及抗炎作用优于全方去掉一味或两味反药组。

Objective To observe the effect of combination of incompatible herb of kansui and glycyrrhiza in modification of prescriptions of Gansui Banxia decoction on pathological morphology of liver of ascites rats under different times of administration. Methods Walker-256 cells were used to make the malignant ascites rat model. The Wistar rats were randomly divided into blank group,model group,furosemide group,once or twice of Gansui Banxia decoction group,once or twice of Gansui Banxia decoction removed glycyrrhiza group,once or twice of Gansui Banxia decoction removed kansui group,once or twice of Gansui Banxia decoction removed glycyrrhiza and kansui group. Once group was gavaged at morning,and twice group was gavaged morning and afternoon,which the total dose was as much as the once group. After14 days＇ feed,the liver of every rat was collected to observe pathomorphological changes,and semi-quantitative ratings was conducted. Results Liver cell damage of model group was very serious,while the onceand twice of Gansui Banxia decoction group was alleviated,and the once group was better than twice group. Compared with the once of Gansui Banxia decoction group,the liver cell damage of once of Gansui Banxia decoction removed glycyrrhiza group,once of Gansui Banxia decoction removed kansui group,once of Gansui Banxia decoction removed glycyrrhiza and kansui group was aggravated. Compared with the twice of Gansui Banxia decoction group,the liver cell damage of twice of Gansui Banxia decoction removed glycyrrhiza group,twice of Gansui Banxia decoction removed kansui group,twice of Gansui Banxia decoction removed glycyrrhiza and kansui group was aggravated. Conclusion The once and twice of Gansui Banxia decoction group has certain improvement effect on liver injury,and the once group is better than twice group; Injury repair and anti inflammatory effect of Gansui Banxia decoction group is beteer than Gansui Banxia decoction removed one or two incompatible herb.