甘遂半夏汤加减甘遂甘草不同给药次数对腹水大鼠水通道蛋白-2基因及肾凋亡的影响

Comparing the effect of Gansui Banxia decoction plus or reduce kansui and glycyrrhiza on AQP-2mRNA expression and apoptosis rate of kidney tissue in ascites rats under different times

目的观察不同给药次数条件下的甘遂半夏汤加减甘遂甘草反药组合对腹水模型大鼠肾脏水通道蛋白(aquaporin,AQP)-2 mRNA表达及肾组织凋亡的影响。方法采用Walker-256细胞制造癌性腹水模型,将Wistar大鼠按体质量随机分为空白组、模型组、阳性对照组、全方给药一次组、去草给药一次组、去遂给药一次组、去草遂给药一次组、全方给药二次组、去草给药二次组、去遂给药二次组、去草遂给药二次组,共计11组。给药一次组每天上午给药一次,给药二次组上午下午各给药一次,一二次组每天给药总量一致。腹水长成14天取材,摘取肾脏,液氮保存,进行PCR和免疫组化实验。结果甘遂半夏汤全方给药一次组和全方给药二次组的AQP-2 mRNA表达及肾组织凋亡无明显差异。但全方组的AQP-2 mRNA表达及肾组织凋亡均较全方去一味或两味反药组有降低趋势。结论含甘遂甘草反药组合的甘遂半夏汤全方给药一次组和给药二次组抑制AQP-2mRNA表达和对癌性腹水造成的肾组织细胞过度凋亡的减缓作用无明显差异,且均优于全方去掉一味或两味反药组。

Objective To find out the effect of Gansui Banxia decoction plus or reduce kansui and glycyrrhiza under different times of administration on AQP-2 mRNA expression and apoptosis rate of kidney tissue in malignant ascites rats. Methods Walker-256 cells were used to induce the malignant ascites rat model.The Wistar rats were randomly divided into blank group,model group,furosemide group,once or twice of Gansui Banxia decoction group,once or twice of Gansui Banxia decoction removed glycyrrhiza group,once or twice of Gansui Banxia decoction removed kansui group,once or twice of Gansui Banxia decoction removed glycyrrhiza and kansui group. Once group was gavaged at morning,and twice group was gavaged at morning and afternoon,which total dose was as much as the once group. After 14 days＇ feed,kidney was collected and kep in liquid nitrogen and then RT-PCR and immunohistochemistry experiments was conducted. Results The result of AQP-2 mRNA expression and apoptosis rate of kidney tissue showed no difference in once and twice Gansui Banxia decoction group,but showed a decreasing tendency compared to the groups removed one or two imcompatible pairs. Conclusion Diuresis of Gansui Banxia decoction is better than it removed one or two imcompatible pairs. Gansui Banxia decoction can reduce the excessive apoptosis of kidney tissue and the effect of whole prescriptionis is better than it removed one or two imcompatible pairs.