摘要
双生病毒是一类世界范围广泛发生的植物单链DNA病毒,其致病危害严重,是作物病害防控的重要对象.但同时,双生病毒具有快速侵染、增殖、转录、表达而不整合入植物基因组的特点,使之成为有广阔发展前景的高效病毒表达载体.本文概括介绍了双生病毒结构和双生病毒载体发展历史,详细归类了双生病毒载体的具体构建方式和应用条件,讨论了双生病毒载体在生物技术应用中的优势,并简要介绍了双生病毒启动子的组织特异性表达特性,为进一步利用双生病毒载体提供了相关背景知识.
Geminiviruses are a group of single-stranded DNA viruses with worldwide distribution. Despite their destructive damage on crop production, geminiviruses can serve as plant virus-based expression vectors for plant genetic engineering due to their ability in efficient infection, replication and transcription without integrating into host plants＇ genome, which makes them potent in transient expression of exogenous genes. This review introduced structures of geminiviruses, the development of geminivirus-based vectors and applications of these vectors. It also described geminiviruse＇ promoters and their tissue-specific activity. In summary, we provide comprehensive information for the application of geminiviruses in biotechnology.
出处
《中国科学：生命科学》
CSCD
北大核心
2016年第5期524-534,共11页
Scientia Sinica(Vitae)
基金
国家自然科学基金(批准号:31390422)资助
