基质金属蛋白酶-9在静脉性溃疡组织中的表达及意义

The expression of matrix metalloproteinase-9 in the chronic venous ulcer tissues and significance

目的 观察静脉性溃疡组织中的基质金属蛋白酶-9（MMP-9）表达,探讨静脉性溃疡血管再生障碍机制.方法 采集我院64例静脉性溃疡患者溃疡创面标本,其中男52例,女12例,平均年龄（56.27±5.12）岁,按病程分为2周组、3周组、4周组、5周组,以创伤性溃疡50例作为对照组,平均年龄（54.42±6.87）岁,Western blot检测各组溃疡创面MMP-9、血管内皮生长因子受体2（VEGFR2）表达趋势;用免疫荧光双染色检测MMP-9/CD31在溃疡组织中表达的相关性.结果 Western blot结果显示,静脉性溃疡MMP-9、VEGFR2变化趋势一致,MMP-9在病程4周、5周组与病程2周、3周组比较（0.181±0.031、0.007±0.014比0.512±0.113、0.685±0.174）,VEGFR2在病程4周、5周组与病程3周组比较（0.614±0.143、0.497±0.262比1.465±0.074）差异均有统计学意义（P＜0.05）,经免疫荧光双染色显示静脉性溃疡组织中MMP-9/CD31的表达下调显著相关.结论 MMP-9调控VEGFR2的表达降低,可能与静脉性溃疡组织局部血管新生障碍有关.

Objective To observe the expression of matrix metalloproteinase-9 （MMP-9） in the tissues of venous ulcer,and to explore the pathological mechanism of angiogenesis dysfunction.Methods Sixty-four ulcer samples were obtained from the patients [（56.27 ± 5.12） years old] including 52 males and 12 females with lower extremity venous ulcer treated in our hospital.The ulcer samples were divided into 2-,3-,4-,and 5-week groups based on the course of the disease,and 50 cases of traumatic ulcer [（54.42 ±6.87） years old] served as the contol group.The expression of MMP-9,and vascular endothelial growth factor receptor 2 （VEGFR2） was analyzed by Western blotting and grey ratio detection.The immunofluorescence double staining was used to observe the correlation of MMP-9/CD31 expression.Results There was significant difference in the grey ratio detection of MMP-9 between 4-,and 5-week groups with 2-,3-week groups （0.181 ± 0.031,0.007 ± 0.014 vs.0.512 ± 0.113,0.685 ± 0.174）;VEGFR2 between 4-,and 5-week groups and 3-week group （0.614 ± 0.143,0.497 ± 0.262 vs.1.465 ± 0.074,P 〈 0.05）.Immunofluorescence double staining revealed that the levels of MMP-9/CD31 was also markedly decreased in the venous ulcer group as compared with those in traumatic ulcer group,and the correlation was observed.Conclusion The low expression of VEGFR2 that is regulated by MMP-9 possibly is associated with angiogenesis dysfunction in the tissues of venous ulcer.