红景天苷对骨折愈合Runt相关转录因子2调控机制研究

Regulatory mechanism of Runt related transcription factor 2 in fracture healing by salidrosid

目的 观察红景天苷在骨折愈合的作用,探讨其在成骨作用中对Runt相关转录因子2（Runx2）通路的调控机制.方法 将40只大鼠分为治疗组和对照组,建立SD大鼠闭合骨折模型,检测血液碱性磷酸酶（ALP）和骨保护素（OPG）浓度,采用微焦点断层扫描（MicroCT）比较两组创伤骨折后的成骨改变;运用实时聚合酶链反应（Real-time PCR）方法检测骨痂组织中Runx2、缺氧诱导因子2A（HIF2A）、Wnt4、Wnt10b、β-连环蛋白（ββ-catenin）、糖原合成酶激酶3（GSK-3ββ）、T细胞因子-1（TCF-1）mRNA表达.结果 骨折后第2、4周,治疗组血清ALP[2周：（335.34±9.23） IU/L,4周：（312.45±10.56） IU/L]和OPG[2周：（40.02±2.90） IU/L,4周：（45.56±3.20）pg/ml]浓度较对照组明显升高（P＜0.05）;治疗组骨小梁生长显著增加（P＜0.05）;骨痂组织中Runx2（2周：1.83±0.11,4周：2.23 ±0.11）、HIF2A（2周：1.37 ±0.12,4周：1.50 ±0.12）、Wnt4（2周：1.78 ±0.10,4周：1.66±0.11）、Wnt10b（2周：1.89 ±0.12,4周：1.92±0.13）、ββ-catenin（2周：1.65 ±0.08,4周：1.72±0.10） mRNA表达均显著增加,GSK-3ββ mRNA表达（2周：0.61 ±0.08,4周：0.56±0.09）显著降低（P＜0.05）.结论 红景天苷能促进成骨,对成骨调控通路Runx2有明显的增强作用.

Objective To compare and evaluate the differences of curative effects and biological mechanisms underlying the posttraumatic bone healing,as well as the study of the regulation of Runt related transcription factor 2 （Runx2） signal pathway mechanism of osteogenesis by using Salidroside.Methods To establish an close osteotomy model of SD rats.40 rats divide into experimental group and control group.Using microCT to evaluate and compare the curative efficacy about the posttraumatic bone healing.Using the method of enzyme linked immunosorbent assay （ELISA）,the serum concentrations of alkaline phosphatase （ALP）,osteoprotegerin （OPG） were assessed.Using the method of real-time quantitative polymerase chain reaction （Real-time PCR）,the mRNA expression levels of Runx2,hypoxia inducible factor （HIF） 2A,Wnt4,Wnt10b,β-catenin,glycogen synthase kinase （GSK）-3β,T-cell factor （TCF）-1 in callus tissue were determined.Results At 2 and 4 weeks after surgery,the callus bone structural parameters of microCT were significantly improved in experimental group compared to that of control group （P 〈 0.05）.In addition,at weeks 2 and 4 after surgery,the mRNA expressions of Runx2 （2 w：1.83 ± 0.11,4 w：2.23±0.11）,HIF2A （2 w：1.37±0.12,4 w：1.50±0.12）,Wnt4 （2w：1.78±0.10,4 w：1.66 ± 0.11）,Wnt10b （2 w：1.89 ± 0.12,4 w：1.92 ± 0.13）,β-catenin （2 w：1.65 ± 0.08,4 w：1.72 ±0.10） were significantly increased,whereas the mRNA expressions of GSK-3ββ （2 w：0.61 ± 0.08,4 w：0.56 ± 0.09） were significantly decreased （P 〈 0.05）.The serum concentrations of ALP [2 w：（335.34±9.23） IU/L,4 w：（312.45 ±10.56） IU/L]P,OPG [2 w：（40.02 ±2.90） IU/L,4 w：（45.56 ± 3.20） pg/ml] were significantly increased （P 〈 0.05）.Conclusion Compared with the Control group,the Salidroside group make an effect to fracture healing.