圆盘状受体2在肝癌组织中的表达及其临床意义

Clinicopathological significance of the expression of discoidin domain receptor 2 in primary hepatocellular carcinoma

目的 探讨圆盘状受体2（DDR2）在原发性肝癌组织中的表达和临床意义.方法 免疫组织化学法检测105例原发性肝癌组织中DDR2的表达;Kendall＆#39;s tau法进行相关性分析;Kaplan-Meier法绘制生存曲线,Log-rank检验分析;Cox比例风险回归模型分析独立预后影响因素.结果 与癌旁组织比较,DDR2在肝癌组织中呈阳性高表达.肝癌组织中DDR2表达水平高低与肝癌肿瘤数量、分化程度、巴塞罗那临床肝癌分期（BCLC）、TNM分期、有无静脉浸润和有无卫星结节等临床病理特征之间显著相关（P＜0.05）,而与患者性别、年龄等其他临床病理特征之间无明显相关（P＞0.05）.Cox回归分析结果显示DDR2高表达是肝癌患者总生存率的独立影响因素[风险比（HR）=2.524;95％可信区间（CI）：1.520-7.176;P ＜0.01],也是患者无复发生存的独立影响因素（HR =3.001;95％ CI：1.724～ 10.280;P ＜0.01）.Kaplan-Meier生存分析表明,与DDR2低表达的患者比较,DDR2高表达的患者总体生存（P＜0.05）和无复发生存均较差（P＜0.05）.结论 DDR2高表达参与原发性肝癌的发生发展,是原发性肝癌的独立预后影响因素.

Objective To investigated the expression of discoidin domain receptor 2 （DDR2） and its clinicopathological significance in primary hepatocellular carcinoma.Methods Immunohistochemistry was employed to detect the expression of DDR2 in primary hepatocellular carcinoma tissues of 105 patients.Correlation between variables was analyzed by Kendall＆#39; tau method.Survival curves were plotted using Kaplan-Meier method with Log-rank test.Cox proportional hazard model was used to evaluate the independent prognostic factors.Difference with P 〈 0.05 was considered as statistically significant.Results Compared with paracancerous tissues the expression level of DDR2 increased in tumor tissues.High expression of DDR2 significantly correlated with the clinical parameters including tumor number,differentiation,Barcelona Clinic Liver Cancer （BCLC） classification,TNM stage,venous invasion,satellite nodules （P 〈 0.05）,and showed no significant correlation with other clinical parameters including age,sex and so on.Cox regression analysis indicated that DDR2 expression was independently influencing factor of overall survival [hazard ratio （HR） =2.524;95% confidence interval （CI）：1.520-7.176;P 〈0.01] and recurrence-free survival （HR =3.001;95% CI：1.724-10.280;P 〈0.01） in liver cancer patients.Kaplan-Meier survival analysis showed that patients with high DDR2 expression had poorer overall survival （P 〈 0.05） and poorer recurrence-free survival （P 〈 0.05） than those with low DDR2 expression.Conclusion High expression of DDR2 took part in the development of primary hepatocellular carcinoma,and was independent prognostic factor in primary liver cancer patients.