牙周炎对小鼠脾脏巨噬细胞表型的影响

Effects of periodontitis on the phenotype and secretion function of macrophages in C57 BL/6J mice model

目的:研究实验性牙周炎模型小鼠的脾脏巨噬细胞表型、分泌功能及相对数量变化,以探究牙周炎对脾脏巨噬细胞的影响。方法:22只22周龄小鼠随机分为真性结扎组(P10d组)和假性结扎对照组(C组),各11只牙周结扎10 d后处死。流式细胞术检测单核巨噬细胞及其M1、M2表型的比例。实时荧光定量PCR法检测脾脏中巨噬细胞相关抗炎型细胞因子IL-1β,抑炎型细胞因子IL-10促炎型mRNA相对表达水平的差异。结果:与C组相比,牙周炎组的M1型巨噬细胞占脾成熟巨噬细胞比例下降,M1/M2比例显著下降,M1型巨噬细胞炎症因子IL-1βmRNA表达水平下降。结论:慢性牙周炎能下调M1型巨噬细胞比例,使巨噬细胞表型M1/M2比例下降,促炎因子IL-1βmRNA表达水平下降。

Objective To explore the splenic macrophages phenotype and secretory function of mice with periodontitis, so as to explore effects of periodontitis on macrophages. Methods 22 mice were randomly divided into periodental ligation group（group P10d） and pseudo periodental ligation control group（group C）, with 11 mice in each group. The experimental periodental ligation on mice lasted for 10 days before they were sacrificed.Flow cytometry was applied to detect the expression of M1 and M2 in mononuclear macrophages. Real-time PCR was applied to detect the relative expression of pro-inflammatory cytokines IL-1β and anti inflammatory cytokines IL-10.Results Compared with the control group C, the proportion of M1 macrophages in the periodontitis group decreased, and the ratio of M1 / M2 was also decreased significantly, and IL-1β mRNA also down-regulated.Conclusions Chronic periodontal infection could down regulate the proportion of M1 macrophages, decrease ratio of M1 / M2 and the expression of inflammatory cytokines IL-1β mRNA.