MICB基因多态性与乳腺癌的易感关联性研究

A Study on the Association between Polymorphism of MICB Gene and Breast Cancer

目的:研究海南汉族人群MICB等位基因的多态性与乳腺癌易感性之间的关联性。方法:采用PCRSSP(PCR sequence-specific primers)和PCR-SBT(PCR sequence-based typing)方法对样本MICB等位基因的多态性进行检测。结果:乳腺癌患者中检出14种MICB等位基因;和对照组相比较,MICB*002和MICB*014等位基因在乳腺癌患者组分布频率较少,MICB*002和MICB*014等位基因可能对乳腺癌不易感(MICB*002:OR=0.31,95%CI:0.19-0.51,Pc<0.05;MICB*014:OR=0.32,95%CI:0.17-0.60,Pc<0.05)。MICB*016和MICB*003等位基因在乳腺癌患者组分布较多;MICB*016和MICB*003等位基因可能对乳腺癌易感(MICB*016:OR=10.68,95%CI:2.52-45.28,Pc<0.05;MICB*003:OR=3.57,95%CI:1.34-9.49,Pc<0.05);MICB*002/002和MICB*014/014基因型可能对乳腺癌不易感(MICB*002/002:OR=0.12,95%CI:0.04-0.36,Pc<0.05;MICB*014/014:OR=0.30,95%CI:0.10-0.89,Pc<0.05)。结论:MICB等位基因的多态性与乳腺癌的易感性之间存在关联性。

Objective: To study the association MICB gene polymorphism and breast cancer in han nationality of Hainan. Methods: PCR-SSP and PCR-SBT were used to analyze the gene polymorphism of MICB. Results: Forty allele genes were found in patients of breast cancer,The frequencies of MICB* 002 and MICB* 014 were lower in breast cancer patients than in healthy controls,although the frequencies of MICB* 016 and MICB* 003 were higher; MICB* 016 and MICB* 003 allele genes may be susceptible to breast cancer( MICB * 016: OR = 10. 68,95% CI: 2. 52-45. 28,Pc <0. 05; MICB * 003: OR = 3. 57,95% CI: 1. 34-9. 49,Pc < 0. 05),and MICB* 002 and MICB* 014 allele genes maybe protected( MICB* 002: OR = 0. 31,95% CI: 0. 19-0. 51,Pc < 0. 05; MICB* 014: OR = 0. 32,95% CI: 0. 17-0. 60,Pc < 0. 05); MICB* 002 /002 and MICB* 014 /014 genotypes maybe protected to breast cancer( MICB* 002 /002:OR = 0. 12,95% CI: 0. 04-0. 36,Pc < 0. 05; MICB* 014 /014: OR = 0. 30,95% CI: 0. 10-0. 89,Pc < 0. 05). Conclusions: MICB allele gene polymorphism may be associated with breast cancer.