鳜鱼消化道黏液细胞和6种酶的组织化学定位

Histochemical Localization of Mucous Cells and Six Types of Enzymes in Digestive Tract of Siniperca Chautsi

采用阿利新蓝-过碘酸雪夫氏(AB-PAS)染色和酶组织化学方法对鳜鱼消化道各部位黏液细胞和6种酶的分布与定位进行了研究。结果显示,黏液细胞可为分为4种类型,食道黏液细胞多数为Ⅲ型和Ⅳ型,未见Ⅰ型和Ⅱ型;胃贲门和胃幽门黏膜上皮仅有Ⅰ型黏液细胞;胃体黏膜上皮则以Ⅲ型细胞为主;幽门盲囊中主要为Ⅱ型细胞;前肠和中肠中Ⅳ型黏液细胞最多,Ⅰ型最少;后肠黏液细胞则以Ⅳ型和Ⅱ型为主。酸性磷酸酶(ACP)主要分布于幽门盲囊和前肠的黏膜上皮;碱性磷酸酶(ALP)主要分布于食道、幽门盲囊和整个肠道黏膜上皮;非特异性酯酶(NSE)主要分布于胃幽门、中肠和后肠黏膜上皮;过氧化物酶(POX)在胃幽门黏膜上皮中活性较高;琥珀酸脱氢酶(SDH)主要分布于胃腺中;腺苷三磷酸酶(ATPase)在消化道各部位均有较多分布。鳜鱼消化道黏液细胞和酶的分布型与其它动物有相似之处,也有其一定的特异性,与消化道不同部位的消化吸收机能相适应。

The distribution and localization of mucous cells and six types of enzymes in the digestive tract of Siniperca chautsi were investigated by AB-PAS( alcian blue and periodic acid schiff's reaction) staining and enzyme histochemical method. The mucous cells could be divided into four types,most of mucous cells in esophagus were type Ⅲ and Ⅳcells,no typeⅠ and Ⅱ cells were found; only typeⅠmucous cells were detected in the mucosal epithelia of stomachus cardiacus and stomachus pyloricus,while the mucous cells in the stomachus corpus were mainly type Ⅲ cells; the mucous cells in pyloric caeca were mainly type Ⅱ cells; most of mucous cells in pre-intestine and mid-intestine were typeⅣ cells,typeⅠcells were the least ones; while the mucous cells in hind-intestine were mainly type Ⅱ and Ⅳ cells.Acid phosphatase( ACP) was mainly distributed in the mucosal epithelia of the pyloric caeca and pre-intestine; Alkaline phosphatase( ALP) mainly existed in the mucosal epithelia of the esophagus,pyloric caeca and the whole intestine;Nonspecific esterase( NSE) was mainly distributed in the mucosal epithelia of the stomachus pyloricus,mid-intestine and hind-intestine. Peroxidase( POX) was detected with higher activity in the mucosal epithelia of stomachus pyloricus.Succinate dehydrogenase( SDH) was mainly located in the gastric gland of stomach. Adenosine triphosphatase( ATPase)was detected with higher activity in the whole digestive tract. The distribution pattern of the mucous cells and enzymes in the digestive tract of Siniperca chautsi was similar to that of other animals,but some specific features have also been observed,which conform with the physiological function in the different parts of digestive tract.