摘要
目的:研究不同抗氧化活性蔬菜汁对大鼠血清代谢组的影响。方法:40只雄性Wistar大鼠,随机分为对照组、藕汁组、油菜汁组和黄瓜汁组,每组10只,单笼饲养。对照组灌胃蒸馏水,蔬菜汁组灌胃相应的蔬菜汁。每只大鼠每天灌胃1次,剂量为5 m L,实验周期为4周。实验结束后,大鼠禁食过夜,球后静脉丛采血,离心分离血清,-20℃保存备用。运用基于核磁共振技术和模式识别技术的代谢组学方法,分析比较不同蔬菜汁灌胃后,大鼠血清代谢变化情况和相关代谢物的差异。结果:得分图显示对照组与各蔬菜汁组之间可以很好地分开,但各蔬菜汁组之间没有明显的界限,说明蔬菜汁改变了大鼠血清的代谢模式。其中,含量升高的物质包括乳酸(δ1.34、δ4.14)、胆碱(δ3.22)、β-羟丁酸(β-hydroxybutyrate,β-HB,δ1.22)和内消旋肌醇(δ3.54)。与对照组比较,灌胃藕汁后,血清中含量升高的物质主要有脯氨酸(δ3.38)、糖区物质(δ3.4、δ4.1),降低的物质主要有脂类(δ0.9、δ1.26、δ1.3);灌胃油菜汁后,血清中升高的物质主要有乙酰乙酸(δ2.14)、脯氨酸(δ3.38)、苏氨酸(δ3.58),降低的物质主要有脂蛋白(δ0.9、δ1.26、δ1.3);灌胃黄瓜汁后,含量升高的物质有苏氨酸(δ3.58),降低的物质主要有脂蛋白(δ0.9、δ1.26、δ1.3)。结论:具有不同抗氧化活性的蔬菜汁,如藕汁、油菜汁和黄瓜汁均可改变大鼠血清的代谢模式,这种作用是通过影响大鼠血清中糖类、脂类和蛋白质的代谢来实现的。
Objective: To observe the effects of vegetable juices with different antioxidant capacities on rat serum metabolome. Methods: Forty male rats were divided randomly into four groups consisting of 10 animals each, including control, lotus root juice(LRJ), rape juice(RJ), and cucumber juice(CJ) groups. The rats from the control and experimental groups were respectively administered with distilled water and vegetable juices intragastrically at a dose of 5 m L/rat once daily for 4 weeks. At the end of the experiment, all the rats were fasted overnight and blood samples were collected from the orbital plexus. The serum was separated and stored at-20 ℃ until analysis. A metabolomic approach based on nuclear magnetic resonance analysis was used to investigate the effects of different vegetable juices on the serum metabolome in rats. Results: Scores Plot demonstrated that the control and vegetable juices groups were separated successfully, while there were no distinctive lines among three vegetable juices groups. After being treated by vegetable juices, the serum metabonome in rats changed significantly. In the vegetable juices groups, lactate(δ1.34, δ4.14), choline(δ3.22), β-hydroxybutyrate(β-HB, δ1.22) and internal compensation inositol increased in comparison to control. After being gavaged with lotus root juice, serum proline(δ3.38) and metabolites of sugar area(δ3.4, δ4.1) rose, while lipids declined(δ0.9, δ1.26, δ1.3). After being intragastrically given rape juices, acetoacetic acid(δ2.14), proline(δ3.38) and threonine(δ3.58) was increased, while lipoprotein(δ0.9, δ1.26, δ1.3) was decreased. In the cucumber juice group, threonine(δ3.58) was increased(δ3.58), while lipoproteins(δ0.9, δ1.26, δ1.3) were decreased. Conclusion: Vegetable juices with different antioxidant capacities such as lotus root juice, rape juice and cucumber juice could lead to changes in the metabolic patterns of serum sugar, lipids and protein in rats.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第11期236-241,共6页
Food Science
基金
达能膳食营养研究与宣教基金项目(DIC2009-09)
关键词
代谢组学
蔬菜
抗氧化性
metabolomics
vegetable
antioxidant capacity