降胆固醇乳酸菌的筛选与鉴定

Screening and Identification of Lactic Acid Bacteria of Degrading Cholesterol

目的:从传统食品中筛选具有降胆固醇功能的乳酸菌菌株,并鉴定。方法:以碳酸钙-MRS培养基从传统食品中筛选乳酸菌。应用改良的降胆固醇筛选培养基,筛选具有较高降胆固醇能力的乳酸菌菌株,并结合菌落形态学、过氧化氢酶试验、明胶液化试验和糖发酵试验鉴定菌株。结果:从传统泡菜中筛选出两株乳酸菌Lp T1和Lp T2,其胆固醇降解率分别达到49.11%和50.03%,经鉴定均为植物乳杆菌。结论:筛选的植物乳杆菌具有较高的胆固醇降解率,为其进一步开发应用奠定了良好基础。

Objective： To screen and identify the lactic acid bacteria of degrading cholesterol from Chinese traditional food. Methods： The degrading cholesterol strains were screened based on the calcium carbonate-MRS medium（Calcium Carbonate-Man Rogosa and Sharp Medium） from Chinese traditional food. They were confirmed by in vitro cholesterol levels. These strains were identified by morphologic observation, catalase reaction, gelatin liquefaction reaction, and sugar fermentation. Results： Two bacterial strains（LpT1 and LpT2） which presented a comparatively high ability of degrading cholesterol was screened. The cholesterol degradation rate of two strains were 49.11% and 50.03%. Two strains were identified as Lactobacillus plantarum. Conclusion： The screened two strains have a high cholesterol degrading rate. This study lays a good foundation for the future industrial use.