姜黄素抑制STAT3磷酸化对乳腺癌细胞增殖及Hsp90α表达的影响

Effect of curcumin on Hsp90α expression and cell proliferation in MCF-7 cells via inhibition of STAT3 phosphorylation

目的探讨姜黄素对乳腺癌细胞增殖和Hsp90α表达的影响及其可能机制。方法以乳腺癌细胞株MCF-7为材料,分别用不同浓度的姜黄素(0、5、10、15、20μmol/L)处理细胞36 h,或以10μmol/L姜黄素分别处理细胞12、24、36、48 h,用MTT法检测细胞生长抑制率,分别采用实时定量PCR和Western blot法检测Hsp90αmRNA、Hsp90α及磷酸化STAT3(p-STAT3)蛋白的表达。结果姜黄素处理MCF-7细胞后,总STAT3蛋白的表达无明显差异,p-STAT3表达明显下调;同时,姜黄素处理组MCF-7细胞代谢MTT的能力降低,细胞生长的抑制率明显上升,Hsp90αmRNA和蛋白质的表达下调,且该效应呈浓度和时间依赖性(P<0.05)。结论姜黄素可能通过STAT3信号途径,调控Hsp90α的表达,进而干预乳腺癌细胞的增殖。

Purpose To investigate the effect of cureumin on the expression of Hsp90α and cells proliferation in MCF-7 cells and its mechanism. Methods The breast cell line MCF-7 was used as the study object, and treated with different concentrations of curcumin （0, 5, 10, 15, 20μmoL/L） for 36 h, or treated with 10 μmol/L eureumin for 12, 24, 36, 48 h, respectively. The cell growth inhibition rate was detected by MTT. Real-time PCR and Western blot assays were used to detect the expression of Hspg0α and STAT3 phosphorylation protein. Results MCF-7 cells were treated by curcnmin, which the expression of total STAT3 showed no significant difference and the expression of p-STAT3 was down-regulated. Meanwhile, in curcumin treatment MCF-7 ceils, MTT metabolic ability was decreased, the rate of cell growth inhibition was increased, and the expression of Hsp90α was down-regulated. These effects were concentration- and time-dependent manner （ P 〈 0. 05 ）. Conclusion Curcumin may regulate the expression of Hsp90α and intervene the proliferation of breast cancer cells through the STAT3 signaling pathway.