有机肥配施氮肥设施菜田FDA水解酶活性与肥力相关性研究

Correlation Between FDA Enzyme Activity and Soil Fertility Under Combining Application Organic and Nitrogen Fertilizer in Facility Vegetable Field

以长期定位施肥设施菜田土壤为研究对象,研究了有机肥配施氮肥条件下土壤荧光素二乙酸(FDA)水解酶与土壤肥力(理化性质、酶活性、微生物等方面)的相关性。结果表明:FDA活性以施有机肥的处理综合表现高于单施氮肥的处理,其中以有机肥+尿素46.12 g表现最为突出;有机肥配施氮肥可使FDA水解酶的活性提高66.2%以上;FDA水解酶活性与土壤理化特性(有机质、速效磷和速效钾含量,含水量)正相关(r>0.9,P<0.05);氮肥投入量影响了FDA水解酶活性与碱解氮和p H的相关性(r<0.4);FDA水解酶活性与土壤脲酶、转化酶、纤维素酶、中性磷酸酶、过氧化氢酶、多酚氧化酶和微生物功能多样性指数(H、S、U)正相关性,仅与优势度指数(D)负相关(0.7<|r|<1);FDA水解酶活性与番茄产量正相关(0.8<r<1,P<0.05)。

An investigation was made on a long-term fertilization facility vegetable field,to study the correlation between soil FDA hydrolysis enzyme and soil fertility（physical and chemical properties,enzyme activities, microbial fertility）. The results showed that organic manure treatment and comprehensive performance was higher than that of single nitrogen fertilizer,especially,manure ＋ urea 46.12 g. Organic fertilizer with nitrogen fertilizer can make the FDA hydrolase activity increased by more than 66.2%. FDA hydrolase activity showed a significant positive correlation with and soil physicochemical properties（organic matter,available phosphorus,available potassium,water content）（r〉0.9,P〈0.05）. The amount of N inputted would effect the correlation between available nitrogen,p H and FDA hydrolase activity,FDA hydrolytic enzyme activity also showed a positive correlation with other soil urease,invertase,cellulase,neutral phosphatase,catalase,polyphenol oxidase and microbial functional diversity indices（H,S,U）,only Simpson index（D）was negative（0.7〈 ｜r｜〈 1）. FDA hydrolase activity was significantly correlated with fruit yield（0.8 〈r〈 1,P〈 0.05）.