紫背天葵高通量转录组测序分析

Sequencing and Analysis of the Transcriptome of Gynura bicolor

为探讨紫背天葵(Gynura bicolor)花青素等物质合成的遗传基础,采用高通量测序技术(Illumina HiSeq 2500)对其嫩叶进行转录组测序,共获得21 387 624个序列读取片段(reads),将测序数据进行序列组装后,获得33 314个单基因簇(Unigene),其中超过1 kb的7 792个Unigene中共检测到2 387个SSR位点。对所得Unigene进行不同数据库注释,22 048和14 417个Unigene分别在Nr和Swiss Prot数据库有同源比对信息,并发现有29个Unigene与花青素合成相关;Pfam功能注释到13 909个Unigene分为5 198类相关蛋白功能区域,有12个Unigene涉及到花青素合成;GO注释到11 613个Unigene分为细胞组分、分子功能及生物学过程等3大类51个功能组,有24个Unigene与花青素合成有关;COG注释到的6 589个Unigene功能系统分为24类,而KOG注释到的13 498个Unigene功能系统分为25类;以KEGG数据库为参考,将4 466个Unigene定位到108个代谢途径分支,其中有47个Unigene与类黄酮生物合成相关。

Gynura bicolor is rich in anthocyanidin and other functional components. In order to explore genetic basis for the synthesis of these active substances,the transcriptome of Gynura bicolor was sequenced by Illumina Hi Seq 2500 platform,a total of 21 387 624 reading fragments were generated which formed 33 314 unigenes by sequence splicing. 2 387 SSR were found in 7 792 unigenes over 1 kb. All unigenes were annotated by different databases. 22 048 and 14 417 unigenes were annotated against Nr and Swiss Prot database respectively,and 29 unigenes were related to anthocyanidin biosynthesis;by Pfam database the 13 909 unigenes were included in 5 198 families of protein functional regions and 12 unigenes were related to anthocyanidin biosynthesis;by GO database the 11 613 unigenes were divided into 3 categories containing 51 function groups and 24 unigenes were related to anthocyanidin biosynthesis;by COG and KOG databases the 6 589 and 13 498 unigenes were grouped into 24 functional categories and 25 ones,respectively;by KEGG database the 4 466 unigenes were divided into 108 metabolism pathways,47 unigenes involved flavonoid biosynthesis.