灵芝孢子粉增强免疫功能的研究

Study on Immune-enhancing Effects of Ganoderma Lucidum Spore Powder

目的研究灵芝孢子粉(破壁)的增强免疫作用。方法通过Con A诱导的小鼠脾淋巴细胞转化实验、绵羊红细胞诱导的小鼠迟发型变态反应实验(足跖容积法)、抗体生成细胞检测实验(Jerne改良玻片法)、血清溶血素测定(血凝法)、小鼠碳廓清实验、小鼠腹腔巨噬细胞吞噬鸡红细胞实验(半体内法)、NK细胞活性实验(乳酸脱氢酶测定法)评价灵芝孢子粉增强免疫功效。结果 Con A诱导的小鼠脾淋巴细胞转化实验、迟发型变态反应实验、抗体生成细胞检测实验、腹腔巨噬细胞吞噬鸡红细胞实验4项实验结果均为阳性。结论灵芝孢子粉(破壁)样品具有增强免疫作用。

OBJECTIVE To investigate the immune-enhancing effects of shell-broken Ganoderma lucidum spore powder. METHODS The immune-enhancing effects were evaluated by Con A-induced mice lymphocyte transformation test, sheep red blood cell-induced delayed-type hypersensitivity（DTH） test（foot volume method）, antibodies formation in spleens（modified Jerne＇s method）, serum hemolysin（blood agglutination method）, carbon clearance test, phagocytic function of mice macrophages against cock erythrocytes（in-half body method）, NK cell activity test（lactate dehydrogenase method）. RESULTS The Con A-induced mice lymphocyte transformation test, DTH test, antibodies formation in spleens test and phagocytic function of mice macrophages against cock erythrocytes test showed positive results. CONCLUSION The Ganoderma lucidum spore powder showes good immune-enhancing effects.