蛇床子素通过p53信号通路诱导人乳腺癌细胞株MCF-7凋亡

Osthole Induce Human Breast Cancer Cells Lines MCF-7 Apoptosis by Activating p53 Signaling Pathway

目的探讨蛇床子素对人乳腺癌细胞株MCF-7的作用及其机制。方法对数生长期的MCF-7细胞用0,25,50,100?mol·L?1的蛇床子素处理。细胞培养48 h后,利用MTT检测细胞增殖;流式检测细胞凋亡和膜电位。RT-PCR检测p53、p21、BCL-2、Bax和Cyt C m RNA水平;免疫印迹法检测p53、p21、BCL-2、Bax和Cyt C表达。结果蛇床子素可以成浓度依赖性诱导MCF-7细胞增殖抑制和凋亡,上调Bax、p53、p21和Cyt C表达,下调Bcl-2的表达以及细胞膜电位。结论蛇床子素可通过激活p53信号通路而诱导MCF-7细胞凋亡。

OBJECTIVE To investigate the effect and mechanisms of the osthole on the human breast cancer cells lines MCF-7. METHODS The human breast cancer cells lines MCF-7 were cultured in vitro and the MCF-7 cell at logarithmic growth phase was treated with different concentration of osthole（0, 25, 50, 100 μmol·L^-1） for 48 h. MTT and flow cytometric analysis were used to evaluate the proliferation, apoptosis, and membrane potential of MCF-7 cells. RT-PCR was used to examnine the p53, p21, BCL-2, Bax and Cyt C m RNA level. The expression of p53, p21, BCL-2, Bax and Cyt C in MCF-7 cell were detected by Western blotting. RESULTS Osthole treatment could induce the proliferation inhibition, apoptosis and the expression of Bax, p53, p21 and Cyt C with decreasing the membrane potential and the expression of BCL-2 on MCF-7 cell in a concentration dependent manner. CONCLUSION Osthole can induce the apoptosis of MCF-7 by activating p53 signaling.