葫芦巴籽和桑叶提取混合物对胰岛素抵抗糖脂代谢紊乱大鼠模型的降血糖作用及其机制研究

Anti-hyperglycemic Effect of Fenugreek Seed and Mulberry Leaf Extract Formula on the Blood Glucose Levels of the Insulin Resistance and Metabolism Disorder Rats Models and the Mechanism

目的探讨葫芦巴籽和桑叶提取混合物(fenugreek seed and mulberry leaf extract,FSML)对胰岛素抵抗糖脂代谢紊乱模型大鼠的降血糖作用及其作用机制。方法建立2个不同的胰岛素抵抗糖脂代谢紊乱大鼠模型,测定大鼠血糖、血脂指标,以及胰岛素受体底物蛋白1(IRS1)、葡萄糖转运蛋白4(GLUT4)、过氧化物酶体增殖物激活受体γ(PPARγ)蛋白的表达。结果对模型1,FSML高、低剂量组均能明显降低模型大鼠的0.5h血糖值及AUC(与模型组比较,P<0.01)。对模型2,FSML高剂量组能显著降低0.5 h血糖值及AUC(与模型组比较,P<0.05)。Western blot实验结果表明,FSML高、低剂量组均能提高GLUT4和PPARγ蛋白的表达(与模型组比较,P<0.05)。结论 FSML具有降血糖作用,其作用机制可能是上调PPARγ蛋白的表达,调节胰岛素信号通路,增加GLUT4蛋白表达,从而增加细胞的葡萄糖摄取,减少外周胰岛素靶组织的胰岛素抵抗。

OBJECTIVE To explore the function of fenugreek seed and mulberry leaf extract（FSML） on regulating the blood glucose level and its related molecular mechanism. METHODS Two rat models of dexamethasone（Model 1） and alloxan（Model 2） were established. The concentration of blood glucose, blood lipids and the protein expression levels of the IRS1, GLUT4 and PPARγ were tested to evaluate its hypoglycemic function. RESULTS In the Model 1, the FSML treatment groups at both two doses had a significant lower 0.5 h blood goucose level and AUC value compared to the Model group（P〈0.05）. In the Model 2, the high dose of FSML treatment group reduced the 0.5 h blood glucose level and AUC value when compared with the model group（P〈0.05）. The results of the Western blot test showed that two FSML treatments groups significantly increased the GLUT4 and PPARγ protein expression levels when compared with the model group（P〈0.05） in Model 1. CONCLUSION This study demonstrates that the FSML has the potential anti-hyperglycemic effect and the possible mechanism is that FSML can regulate the insulin signal pathway through up-regulating the protein expression of PPARγand increase the protein expression of GLUT4, accordingly the cellular blood glucose uptake is increased and the insulin resistance in the muscular and adipocyte tissues is decreased.