中药饮片麦冬和芫花中耐热菌的考察

Investigation of Thermoduric Bacteria in Chinese Herbal Medicine:Ophiopogonis Radix and Genkwa Flos

目的考察中药饮片麦冬和芫花中耐热菌的种类和数量,并探索在冷藏储存过程中的变化情况。方法采用中国药典2015年版方法测定不同沸水浴时间的耐热菌数,并对120 min沸水浴处理后存活耐热菌采用16S r DNA基因序列分析方法进行菌种鉴定,同时考察了冷藏储存一定时间后耐热菌数的变化。结果随着沸水浴时间的增加,耐热菌数不断减少。芫花即使经过120 min沸水浴处理后仍含有较高数量的耐热菌,且经48 h冷藏储存后,耐热菌数量出现明显增加。耐热菌株鉴定结果均为芽孢杆菌属。结论从用药安全性角度考虑,应控制中药饮片中的耐热菌,并关注中药代煎服务存在的微生物污染问题。

OBJECTIVE To investigate the thermoduric bacteria species and contamination in Ophiopogonis Radix and Genkwa Flos, explore the influence on the thermoduric bacteria by refrigerated storage. METHODS According to Chinese Pharmacopoeia（2015 edition）, the amounts of the thermoduric bacteria were identified after different boiling bath time, and the species of thermoduric bacteria were identified by 16 s r DNA gene sequence test system. To investigate the variation of the thermoduric bacteria by refrigerated storage at the same time. RESULTS By increasing the boiling bath time, the amounts of thermoduric bacteria were reduced. The thermoduric bacteria were still at high quantity even after 120 min boiling bath in Genkwa Flos, and after 48 h refrigerated storage, the amounts of thermoduric bacteria were significantly increased. The thermoduric bacteria were subtilis. CONCLUSION For safety pledge, it is recommended to control thermodurie bacteria in the Chinese herbal medicine, and pay attention to the microbial contamination of TCM decoction service.