青蒿烯对人宫颈癌Hela细胞凋亡的影响及作用机制

Effect of artemisitene on apoptosis of cervcal cancer cell line Hela

目的:研究青蒿烯对宫颈癌Hela细胞凋亡的影响,并初步探讨青蒿烯诱导Hela细胞凋亡的作用机制。方法:通过MTT法分析不同浓度青蒿烯对Hela细胞增殖活力的影响;运用Hoechst33258荧光染色法和流式细胞术检测青蒿烯对Hela细胞诱导凋亡的影响;应用caspase-3/7活性检测试剂盒检测青蒿烯处理后Hela细胞caspase-3/7活性的变化情况;Western blot法检测caspase-3/7,Fas-L和Fas蛋白水平的变化。结果:与对照组相比,青蒿烯抑制Hela细胞的增殖(P<0.05,P<0.01),且显著地诱导Hela细胞凋亡(P<0.01);青蒿烯促进aspase3/7的活化,增加caspase-3/7的切割以及Fas-L的表达。结论:青蒿烯具有诱导宫颈癌Hela细胞凋亡的作用,这一作用可能与青蒿烯上调Fas-L的表达,激活死亡受体信号通路有关。

Objective To investigate the pro-apoptotic activity of artemisitene on cervical cancer cell line Hela and its mechanisms. Methods The inhibitory effect of artemisitene on the proliferation of Hela cells was measured by MTT assay. Hoechst33258 fluorescence staining and flow cytometry were employed to determine the pro-apoptotic effect of artemisitene on Hela cells. The activity of caspase-3 / 7 was analyzed by caspase-3 / 7activity assay kit. Westren blot was used to detect the protein levels of cleaved caspase-3 / 7, Fas-L and Fas.Results MTT assay showed that artemisitene inhibited Hela cells proliferation（P〈0.05,P〈0.01）. Moreover,Hela cells were significantly induced to apoptosis by artemisitene. Accordingly, the enhanced activity and increased cleaved form of caspase-3 / 7 were observed in artemisitene-treated group. In addition, artemisitne promoted the expression of Fas-L. Conclusion Artemisitene significantly induced apoptosis of Hela cells. The effect may be related to the up-regulation of Fas-L, and subsequent activated death receptor apoptotic signaling pathway.