高危型人乳头状瘤病毒16/18在喉癌中的感染及整合状态

The infection and integration status of high-risk human papillomavirus 16 / 18 in laryngeal cancer

目的:探讨高危型人乳头状瘤病毒(HPV)16/18型感染及整合状态对广东地区喉癌发病的意义。方法:应用实时定量聚合酶链反应(PCR)检测2010年1月至2012年6月广东省人民医院耳鼻咽喉头颈外科喉癌石蜡包埋标本中HPV16/18 E2、E6的拷贝数,分析HPV16/18病毒感染情况和整合状态,并对其进行随访。结果:82例喉癌标本中,2例HPV16阳性(2.4%),整合状态为游离型或混合型,未发现HPV完全整合入宿主DNA者。HPV阳性2例患者存活至今无复发。结论:高危型HPV16/18在喉癌中的感染率较低,HPV16/18可能不是广东地区喉癌的主要发病因素,对喉癌的预后影响可能有限。

Objective To investigate the significance of the infection and integration status of high-risk human papillomavirus（HPV）16 / 18 in patients with laryngeal cancer in Guangdong province. Methods The copy number of HPV16 / 18 E2,E6 were detected by quantitative real-time polymerase chain reaction（PCR） for the analysis of the relationship of HPV16 / 18 infection and integration status in formalin-fixed paraffin-embedded laryngeal cancer samples in Guangdong General Hospital from January of 2010 to June of 2012.All patients were followed up. Results HPV16 was positive in 2 out of 82 specimens（2.4%） and HPV18 was negative in all specimens. All positive cases are HPV16 infection, with integrated form manifesting episomal or mixed type. No HPV were found to integrate into host DNA. All HPV positive patients survived without relapse up till now.Conclusion High-risk HPV16 / 18 infection rate was low in laryngeal cancer, HPV may not be a main causal factor in laryngeal cancer carcinogenesis in Guangdong province and it may hardly affect the prognosis of patients with laryngeal cancer.