miRNA-146a rs2910164位点的单核苷酸多态性与原发性肝癌术后复发的相关性

Association between single nucleotide polymorphism of microRNA-146a and recurrence after liver cancer surgery

目的探讨miRNA-146a rs2910164位点的单核苷酸多态性与肝癌术后复发的相关性。方法选取于东风医疗集团茅箭医院行肝癌根治性切除术的89例原发性肝癌患者,按照术后是否复发分为复发组和非复发组。应用Taq Man探针法对miRNA-146a rs2910164 G/C位点的基因分型,比较两组各基因型的频率及miRNA-146a的单核苷酸多态性与肝癌术后复发风险的关系。计量资料组间比较采用成组t检验,计数资料采用χ2检验,并采用多因素Logistic回归分析可能影响肝癌复发的因素。结果非复发组miRNA-146a G/C三种基因型符合Hardy-Weinberg平衡定律(P>0.05)。肝癌术后复发组和非复发组miRNA-146a G/C基因型频率差异有统计学意义(χ2=9.115,P=0.010),其中复发组miRNA146a(rs2910164)CG基因型频率明显高于非复发组(χ2=4.013,P=0.039),GG基因型频率明显低于非复发组(χ2=9.046,P=0.003)。多因素Logistic分析显示肿瘤直径、miRNA146a(rs2910164)CG基因型是肝癌术后复发的危险因素(OR=1.075,P=0.003 9;OR=6.215,P=0.001 4),miRNA146a(rs2910164)GG基因型是肝癌术后复发的保护因素(OR=0.382,P=0.002 5)。结论 miRNA-146a rs2910164 C/G位点的多态性与肝癌根治术后复发相关,rs2910164位点的CG基因型可能是引起肝癌患者术后复发的危险因素。

Objective To investigate the association between the single nucleotide polymorphism（ SNP） of miRNA- 146 a rs2910164 and recurrence after liver cancer surgery. Methods A total of 89 patients with primary liver cancer who underwent radical resection for liver cancer in Maojian Hospital of Dongfeng Medical Group were enrolled,and according to the presence or absence of postoperative recurrence,they were divided into recurrence group and non- recurrence group. The Taq Man probe method was used to determine the genotypes（ G / C）of miRNA- 146 a rs2910164. The frequency of each genotype was compared between the two groups to investigate the association between the SNP of MicroRNA- 146 a and recurrence after liver cancer surgery. The independent- samples t test was used for comparison of continuous data between the two groups,the chi- square test was used for comparison of categorical data between the two groups,and multivariate logistic regression analysis was used to determine the factors associated with the recurrence of liver cancer. Results The three genotypes of miRNA- 146 a G / C in non- recurrence group conformed to the Hardy- Weinberg equilibrium law（ P〉0. 05）. After the surgery for liver cancer,the frequency of miRNA- 146 a G / C genotype showed a significant difference between the recurrence group and the non- recurrence group（ χ2= 9. 115,P = 0. 010）,and compared with the non- recurrence group,the recurrence group had a significantly higher frequency of miRNA- 146a（ rs2910164） CG genotype（ χ2= 4. 013,P = 0. 039） and a significantly lower frequency of GG genotype（ χ2= 9. 046,P = 0. 003）. The multivariate logistic regression analysis showed that tumor diameter（ OR = 1. 075,P = 0. 003 9） and miRNA- 146a（ rs2910164） CG genotype（ OR = 6. 215,P = 0. 001 4） were the risk factors for recurrence after the surgery for liver cancer,and that miRNA146a（ rs2910164） GG genotype was the protective factor against recurrence after the surgery for liver cancer（ OR = 0. 382,P = 0. 002 5）.Conclusion The SNP of MiRNA- 146a（ rs2910164 C / G） is associated with recurrence after the radical surgery for liver cancer,and the CG genotype of rs2910164 may be the risk factor for postoperative recurrence after the surgery for liver cancer.